Immunotherapy and diagnosis of mucormycosis using CotH

• US 9,279,002 B2
• Filed: 09/14/2012
• Issued: 03/08/2016
• Est. Priority Date: 09/15/2011
• Status: Active Grant

• Current Assignee: Los Angeles Biomedical Research Institute At Harbor-Ucla Medical Center (University of California)
• Original Assignee: Los Angeles Biomedical Research Institute At Harbor-Ucla Medical Center (University of California)
• Inventors: Ibrahim, Ashraf S., Liu, Mingfu, Ghebremariam, Teklegiorgis, Fu, Yue, Edwards, John E., Filler, Scott
• Primary Examiner(s): Zeman, Robert A
• Application Number:
  US13/620,563
• Publication Number:
  US 20130108642A1
• Time in Patent Office: 1,271 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: A61K 2039/505 comprising antibodies
• CPC Class Codes: A61P 31/10 Antimycotics
• CPC Class Codes: C07K 14/37 from fungi
• CPC Class Codes: C07K 16/14 against material from fungi, algea or lichens
• CPC Class Codes: C07K 2317/24 containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
• CPC Class Codes: C07K 2317/34 Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
• CPC Class Codes: C07K 2317/622 Single chain antibody (scFv)
• CPC Class Codes: C07K 2317/76 Antagonist effect on antigen, e.g. neutralization or inhibition of binding
• CPC Class Codes: G01N 2333/37 from fungi
• CPC Class Codes: G01N 2469/10 Detection of antigens from microorganism in sample from host
• CPC Class Codes: G01N 33/56961 Plant cells or fungi

Abstract

The invention provided Mucorales CotH polypeptides and encoding nucleic acid molecules. The Mucorales CotH polypeptides and encoding nucleic acids can be advantageously used to diagnose, treat or prevent fungal conditions, in particular mucormycosis.

Claims

1. An isolated anti-Mucorales CotH antibody that abrogates endocytosis of a fungus belonging to the species Rhizopus oryzae by a mammalian cell, wherein the antibody specifically binds to a Mucorales CotH polypeptide consisting of the amino acid sequence of SEQ ID NO:

39 or SEQ ID NO;

40.

2. A pharmaceutical composition comprising a pharmaceutically acceptable carrier and the anti-Mucorales CotH antibody of claim 1.

3. The antibody of claim 1, wherein said antibody is a monoclonal antibody.

4. The antibody of claim 1, wherein said antibody is a polyclonal antibody.

5. The antibody of claim 1, wherein the antibody is a non-naturally occurring antibody.

6. The antibody of claim 5, wherein the antibody is a chimeric antibody.

7. The antibody of claim 5, wherein the antibody is a humanized antibody.

8. The antibody of claim 5, wherein the antibody is a single chain antibody.

9. The antibody of claim 5, wherein the antibody comprises a detectable marker or label.

10. The antibody of claim 5, wherein the antibody is an antibody fragment that retains the binding specificity to the Mucorales CotH polypeptides consisting of the amino acid sequence of SEQ ID NO:

39 or SEQ ID NO;

40.