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Nucleic acid processing kits and devices

  • US 9,279,121 B2
  • Filed: 11/10/2008
  • Issued: 03/08/2016
  • Est. Priority Date: 05/19/2003
  • Status: Expired due to Fees
First Claim
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1. A method for enzymatically synthesizing cDNA from a biological sample, comprising:

  • (a) combining said biological sample with a disruption reagent in a container to produce a mixture comprising at least 2M chaotropic agent;

    (b) diluting said mixture with at least one aqueous reagent to reduce the concentration of said chaotropic agent to less than 0.05 M; and

    (c) incubating at least a portion of said mixture with reverse transcriptase.

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