Methods for isolating bacteria

• US 9,279,134 B2
• Filed: 09/18/2013
• Issued: 03/08/2016
• Est. Priority Date: 01/19/2009
• Status: Active Grant

• Current Assignee: Universite De Montpellier I
• Original Assignee: Centre National De La Recherche Scientifique, Deinove
• Inventors: Leonetti, Jean-Paul, Texier, Stphanie
• Primary Examiner(s): Navarro, Albert
• Assistant Examiner(s): Portner, Ginny
• Application Number:
  US14/030,504
• Publication Number:
  US 20140017724A1
• Time in Patent Office: 902 Days
• Field of Search: 424/93.4, 435/71.3
• US Class Current: 1/1
• CPC Class Codes: A23L 29/065 Microorganisms addition of bacteria for nutritional purposes A23L33/135
• CPC Class Codes: A23L 33/10 using additives addition of substantially indigestible substances A23L33/21
• CPC Class Codes: A23L 33/135 Bacteria or derivatives thereof, e.g. probiotics
• CPC Class Codes: A61K 35/741 Probiotics probiotic yeast, e.g. saccharomyces A61K36/06
• CPC Class Codes: A61P 31/04 Antibacterial agents
• CPC Class Codes: A61P 31/10 Antimycotics
• CPC Class Codes: A61P 31/12 Antivirals
• CPC Class Codes: A61P 33/00 Antiparasitic agents
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C07G 11/00 Antibiotics
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
• CPC Class Codes: C12P 1/04 by using bacteria
• CPC Class Codes: C12P 23/00 Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes containing heterorings C12P17/00
• CPC Class Codes: C12Q 1/025 for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics antimicrobial activity C12Q1/18
• CPC Class Codes: C12R 2001/01 Bacteria or Actinomycetales ; using bacteria or Actinomycetales
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The present invention relates to compositions and methods to identify novel bacteria and metabolites derived therefrom. More specifically, the invention describes a novel method to isolate bacteria producing metabolites of interest from environmental samples. Particularly, the invention discloses a method to select rare antibiotic producing bacteria. The invention can be used from any sample and allows the isolation of bacteria having e.g., pharmaceutical or agrochemical interest.

Claims

1. A method for producing an antibiotic, the method comprising (i) culturing, in a suitable medium, a Deinococcus bacterium which naturally produces said antibiotic or an intermediate thereof, (ii) collecting or purifying the antibiotic or intermediate from the culture, and (iii) when an intermediate is produced in step (i), converting said intermediate into said antibiotic.

2. The method of claim 1, wherein the bacterium is a wild-type Deinococcus bacterium.

3. The method of claim 1, wherein the bacterium is a recombinant Deinococcus bacterium.

4. The method of claim 1, wherein the bacterium is cultured in a defined medium.

5. The method of claim 1, wherein the bacterium is cultured in a complex medium.

6. The method of claim 1, wherein the bacterium is cultured at a pH comprised between 4 and 8.

7. The method of claim 1, wherein the bacterium is cultured in a fermentation device or as a continuous culture.

8. The method of claim 1, wherein the antibiotic is chemically modified after collection or purification.