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Laser microdissection method and apparatus

  • US 9,279,749 B2
  • Filed: 05/05/2014
  • Issued: 03/08/2016
  • Est. Priority Date: 09/09/2004
  • Status: Active Grant
First Claim
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1. A method for laser microdissection comprising the steps of:

  • providing a first substrate having an upper surface;

    providing a membrane having an upper surface and a lower surface;

    applying a layer of a biological material to upper surface of the membrane;

    placing the membrane in contact with the first substrate such that the lower surface of the membrane contacts the upper surface of the first substrate;

    providing a second substrate having a surface with a transfer film disposed thereon, wherein the transfer film has adhesive characteristics upon activation by electromagnetic energy;

    identifying at least one targeted portion of the biological material to be microdissected;

    bringing the transfer film into juxtaposition with the first substrate on the side of the biological material in the location of the at least one targeted portion of the biological material;

    activating a first laser source so as to describe at least one closed or substantially closed path around the at least one targeted portion of the biological material to be microdissected, the first laser source eroding the biological material along the described path and defining an interior portion of the biological material and an exterior portion of the biological material;

    activating a second laser source and directing the second laser source at the interior portion of the biological material so as to activate at least one region of the transfer film so that the at least one activated region of the transfer film adheres to the interior portion of the biological material; and

    separating the transfer film and the at least one adhered targeted portion of the biological material from the first substrate.

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