Apparatus, method, system for the determination of the aggregation rate of red blood cells

• US 9,279,800 B2
• Filed: 02/10/2014
• Issued: 03/08/2016
• Est. Priority Date: 01/13/2012
• Status: Active Grant

• Current Assignee: Alcor Scientific Incorporated
• Original Assignee: Alcor Scientific Incorporated
• Inventors: Sacchetti, Peter, Frappa, Francesco
• Primary Examiner(s): Kosson, Rosanne
• Application Number:
  US14/176,307
• Publication Number:
  US 20140154733A1
• Time in Patent Office: 757 Days
• US Class Current: 1/1
• CPC Class Codes: G01N 15/05 in blood
• CPC Class Codes: G01N 2015/0092 Monitoring flocculation or agglomeration
• CPC Class Codes: G01N 2015/012 Red blood cells
• CPC Class Codes: G01N 21/51 inside a container, e.g. in an ampoule G01N21/53 takes precedence
• CPC Class Codes: G01N 33/4905 Determining clotting time of blood by chemical methods G01N33/86, C12Q1/56
• CPC Class Codes: G01N 33/4915 using flow cells flow cytometry G01N15/14

Abstract

The present invention generally relates to an apparatus, method, system for the determination of the aggregation rate of red blood cells. More specifically, the invention concerns a method, system, and the relative apparatus used to determine the aggregation rate of red blood cells, and other parameters related to these, such as viscosity, deformability, elasticity, density, in the field of in vitro medical analyses, using optical systems after or during inducted forces for red blood cell disruption and redistribution generated by ultrasound waves.

Claims

1. An apparatus for determining the aggregation rate of red blood cells, comprising:

a) a hydraulic circuit having a pump for transporting a blood sample portion comprising red blood cells that have aggregated;

b) a reading cell container operationally connected to the hydraulic circuit configured for receipt of the blood sample portion;

c) a light emitter source positioned about the reading cell to pass light through the blood sample portion;

d) an optical receiver positioned opposite the light emitter source and about the reading cell to detect light passing through the blood sample portion;

e) a disruption mechanism connected to the reading cell container for disruption of the red blood cell aggregates within the blood sample portion to assist in recording a disruption rate; and

f) a main controller operationally connected to the disruption mechanism, the main controller configured to activate the disruption mechanism for the disruption of the red blood cell aggregates within the blood sample portion until light detected stops decreasing, indicating the disruption of aggregates within the blood sample portion,

whereby, in a method of using the apparatus, after the disruption mechanism is stopped, the main controller records an aggregation rate of the red blood cells of the blood sample portion for a predetermined time based upon detected light variation.

2. The apparatus of claim 1, further comprising:

a fluid reservoir connected to the reading cell container for receipt of an evacuated blood sample portion from the reading cell container.

3. The apparatus of claim 1, wherein the apparatus comprises an evacuation mechanism to evacuate the evacuated blood sample portion from the reading cell container.

4. The apparatus of claim 3, wherein the evacuation mechanism is configured to provide ultrasound stress to the reading cell container.

5. The apparatus of claim 1, wherein the apparatus determines an aggregation index of the red blood cells for a subsequent estimation of an erythrocyte sedimentation rate, the determination steps being comparable to those in a Westergren method.

6. The apparatus of claim 1, wherein the apparatus determines a disruption index of the red blood cells as rheological parameters usable for pathologic detection purposes.

7. The apparatus of claim 1, wherein the apparatus determines a mean red blood cells shape recovery ability.

8. The apparatus of claim 1, wherein the apparatus determines the plasma viscosity.

9. The apparatus of claim 1, wherein the reading cell container is configured for the optical detection of aggregation reaction.

10. The apparatus of claim 1, wherein the disruption mechanism is an actuator.

11. The apparatus of claim 1, wherein the disruption mechanism is an ultrasonic inducted mechanism for the disruption of red blood cells aggregates.

12. The apparatus of claim 1, wherein the disruption mechanism is electric power applied to a piezoceramic material which is initially ramped up to a level where cell emulsification is detected through the optical reading cell.

13. A method of measuring the disruption rate and the aggregation rate of a sample of red blood cells, comprising:

a) obtaining a blood sample portion comprising aggregated red blood cells;

b) providing the apparatus of claim 1;

c) delivering the blood sample portion into the reading cell container;

d) disrupting the aggregates in the blood sample portion within the reading cell container to distribute and re-suspend the red blood cells;

e) passing light through the blood sample portion within the reading cell container;

f) receiving and detecting light emitted from the reading cell container;

g) recording a disruption rate of the red blood cell aggregates within the blood sample portion based upon the light variation;

h) continuing the disruption of the blood sample portion within the reading cell until the variation in light detected stops decreasing, indicating the disruption of the red blood cell aggregates within the blood sample portion;

i) recording an aggregation rate of the red blood cells in the blood sample portion for a predetermined time based upon detected light variation; and

j) evacuating the blood sample portion from the reading cell container.

14. The apparatus of claim 1, wherein the apparatus determines an aggregation index of the red blood cells for subsequent estimation of an erythrocyte sedimentation rate.

15. The method of claim 14, wherein disrupting the blood sample portion comprises applying electric power to a piezoceramic material initially ramped up to a level where cell emulsification is detected through the optical reading.

16. An apparatus for determining the aggregation rate of red blood cells, comprising:

a) a hydraulic circuit for transporting a blood sample portion comprising aggregated red blood cells;

b) a reading cell container configured to receive the blood sample portion from the hydraulic circuit;

c) a light emitter source to pass light through the blood sample portion while the blood sample portion is in the reading cell container;

d) an optical receiver positioned to detect light passing from the light emitter source through the blood sample portion while the blood sample portion is in the reading cell container;

e) a disruption mechanism connected to the reading cell container to disrupt the aggregated red blood cells within the blood sample portion while the blood sample portion is in the reading cell container; and

f) a main controller configured to selectively activate the disruption mechanism and record an aggregation rate of the red blood cells in the blood sample portion based upon detected light variation.

17. The apparatus of claim 16, further comprising:

g) a fluid reservoir connected to the reading cell container for receipt of a blood sample portion evacuated from the reading cell container; and

h) an evacuation mechanism to evacuate the blood sample portion from the reading cell container, wherein the evacuation mechanism is configured to provide ultrasound stress to the reading cell container.

18. The apparatus of claim 16, wherein the main controller determines the aggregation rate based upon for a predetermined time of disruption.

19. The apparatus of claim 16, wherein the main controller determines the aggregation rate based upon data collected from a start of disruption until light detected stops decreasing.

20. An apparatus for determining the aggregation rate of red blood cells, comprising:

a) a reading cell container configured to receive a blood sample portion comprising aggregated red blood cells from a hydraulic circuit;

b) a light emitter source to pass light through the blood sample portion while the blood sample portion is in the reading cell container;

c) an optical receiver positioned to detect light passing from the light emitter source through the blood sample portion while the blood sample portion is in the reading cell container;

d) a disruption mechanism connected to the reading cell container to disrupt the aggregated red blood cells within the blood sample portion while the blood sample portion is in the reading cell container; and

e) a main controller configured to record an aggregation rate of the red blood cells in the blood sample portion based upon detected light variation.