Methods for tissue analysis

• US 9,282,931 B2
• Filed: 10/03/2011
• Issued: 03/15/2016
• Est. Priority Date: 10/30/2000
• Status: Expired due to Fees

• Current Assignee: The General Hospital Corporation (Mass General Brigham, Inc.)
• Original Assignee: The General Hospital Corporation (Mass General Brigham, Inc.)
• Inventors: Tearney, Guillermo J., Bouma, Brett E.
• Primary Examiner(s): Smith, Ruth S
• Application Number:
  US13/251,594
• Publication Number:
  US 20120022381A1
• Time in Patent Office: 1,625 Days
• US Class Current: 1/1
• CPC Class Codes: A61B 1/00082 Balloons
• CPC Class Codes: A61B 1/0615 for radial illumination
• CPC Class Codes: A61B 1/07 using light-conductive means, e.g. optical fibres
• CPC Class Codes: A61B 5/0066 Optical coherence imaging
• CPC Class Codes: A61B 5/0068 Confocal scanning
• CPC Class Codes: A61B 5/0075 by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy A61B5/0071 takes precedence
• CPC Class Codes: A61B 5/0084 for introduction into the body, e.g. by catheters A61B5/1459 takes precedence
• CPC Class Codes: A61B 5/02007 Evaluating blood vessel condition, e.g. elasticity, compliance
• CPC Class Codes: A61B 5/6885 Monitoring or controlling sensor contact pressure
• CPC Class Codes: A61B 5/7207 of noise induced by motion artifacts
• CPC Class Codes: A61B 5/7257 using Fourier transforms
• CPC Class Codes: G01N 21/49 within a body or fluid

Abstract

The invention relates to methods and systems to optically analyze samples such as tissue based on speckle patterns of microscopic motion, such as Brownian motion.

Claims

1. A method of analyzing a tissue structure, comprising:

illuminating the tissue structure with at least one of a coherent light or a partially coherent light from a light source onto the tissue structure;

receiving a further light reflected from the tissue structure and forming a series of speckle patterns based on the further light; and

obtaining speckle pattern data from the series of speckle patterns at time intervals sufficient to measure a microscopic motion within the tissue structure or within a tissue adjacent to the structure; and

assessing the tissue structure by analyzing spatial characteristics of the speckle pattern data, and determining at least one of structural characteristics or biomechanical characteristics of the tissue structure which is in vivo tissue based on the assessment of the tissue structure.

2. The method of claim 1, wherein the microscopic motion is at least one of a Brownian motion or a motion of cells or cellular organelles.

3. The method of claim 1, further comprising providing a device which includes the light source in direct contact with the tissue structure.

4. The method of claim 1, further comprising providing a detector which is configured to detect a far field speckle from the further light, and which is located farther than one wavelength of light from the tissue structure.

5. The method of claim 1, wherein further comprising providing a detector which is configured to detect a near field speckle from the further light, and which is located within one wavelength of light from the tissue structure.

6. The method of claim 1, wherein the assessing procedure comprises comparing each of the speckle patterns to a series of reference speckle patterns, and quantifying temporal correlation differences between the speckle patterns and the reference patterns.

7. The method of claim 6, wherein the assessing procedure further comprises digitizing each of the speckle patterns, and the quantifying subprocedure comprises evaluating a cross-correlation between the speckle patterns and the reference patterns.

8. The method of claim 7, wherein the assessing procedure further comprises determining a decorrelation rate for the speckle patterns.

9. The method of claim 6, wherein the assessing procedure further comprises digitizing each of the speckle patterns, and the quantifying substep comprises evaluating a maximum cross-correlation between the speckle patterns and the reference patterns.

10. The method of claim 1, wherein the assessing procedure further comprises analyzing spatial characteristics of the speckle patterns to determine structural characteristics of the tissue structure.

11. The method of claim 10, wherein the illuminating procedure comprises illuminating multiple locations of the tissue structure in succession, the receiving procedure comprises forming a separate series of speckle patterns for each respective location of the tissue structure, and the analyzing procedure comprises analyzing each separate series of the speckle patterns and comparing the separate series to determine structural differences between the respective locations of the tissue structure.

12. The method of claim 1, wherein the assessing of the tissue is performed by a computer structural arrangement that analyzes the changes in the speckle patterns at time intervals sufficient to measure the changes caused by the microscopic motion of the objects.

13. The method of claim 1, wherein the assessing step includes assessing the tissue structure that is plaque.

14. The method of claim 1, wherein the assessing step includes assessing the tissue structure that is a blood vessel.

15. The method of claim 1, wherein the assessing step includes assessing the tissue structure that is a coronary artery.

16. The method of claim 1, the assessing of the tissue is performed by a computer structural arrangement that receives data associated with heart beats.

17. A method for analyzing tissue, comprising:

illuminating the tissue with at least one of a coherent light or a partially coherent light from a light source onto the tissue;

receiving a further light reflected from the tissue and form a series of speckle patterns based on the further light; and

analyzing changes in the speckle patterns at time intervals sufficient to measure changes caused by a microscopic motion of objects within the tissue which is in vivo tissue.