Passive microfluidic metering device

• US 9,283,560 B2
• Filed: 11/22/2013
• Issued: 03/15/2016
• Est. Priority Date: 11/22/2013
• Status: Active Grant

• Current Assignee: Sharp Life Science (EU) Limited
• Original Assignee: Sharp Kabushiki Kaisha (Hon Hai Precision Industry Co., Ltd.)
• Inventors: Dothie, Pamela Ann
• Primary Examiner(s): Alexander, Lyle
• Assistant Examiner(s): Wecker, Jennifer
• Application Number:
  US14/087,506
• Publication Number:
  US 20150147777A1
• Time in Patent Office: 844 Days
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/0605 Metering of fluids
• CPC Class Codes: B01L 2200/0626 using levitated droplets
• CPC Class Codes: B01L 2300/0627 Sensor or part of a sensor is integrated
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2400/0478 pistons
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0622 distribution valves, valves having multiple inlets and/or outlets, e.g. metering valves, multi-way valves
• CPC Class Codes: B01L 2400/0683 mechanically breaking a wall or membrane within a channel or chamber
• CPC Class Codes: B01L 2400/0694 vents used to stop and induce flow, backpressure valves
• CPC Class Codes: B01L 2400/086 using baffles or other fixed flow obstructions
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502723 characterised by venting arrangements
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: G01N 15/1404 Handling flow, e.g. hydrodynamic focusing
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

An integrated fluidic device comprising includes an input chamber that provides an input of a sample fluid, and a first overspill chamber in fluid communication with the input chamber. A metering conduit is in fluid communication with the fluid input chamber and the first overspill chamber. The metering conduit meters a first metered volume of fluid from the sample fluid, and the first overspill chamber receives fluid in excess of the first metered volume of fluid. A second overspill chamber is in fluid communication with the metering conduit. The metering conduit meters a second metered volume of fluid from the first metered volume of fluid, and the second overspill chamber receives fluid from the first metered volume of fluid in excess of the second metered volume of fluid. The second overspill chamber has a fluid actuated closable valve for controlling the metering of the second metered volume of fluid.

Claims

1. An integrated fluidic device comprising:

a sample fluid input chamber configured to provide an input of a sample fluid;

a first overspill chamber in fluid communication with the fluid input chamber;

a metering conduit in fluid communication with the fluid input chamber and the first overspill chamber, wherein the metering conduit is configured to meter a first metered volume of fluid from the sample fluid, and the first overspill chamber is configured to receive fluid from the sample fluid in excess of the first metered volume of fluid; and

a second overspill chamber in fluid communication with the metering conduit, wherein the metering conduit is configured to meter a second metered volume of fluid from the first metered volume of fluid, and the second overspill chamber is configured to receive fluid from the first metered volume of fluid in excess of the second metered volume of fluid;

wherein the second overspill chamber has a fluid actuated closable valve configured to isolate the second metered volume of fluid out of the first metered volume of fluid when the fluid actuated closable valve closes; and

wherein the first overspill chamber includes a first absorbent pad configured to absorb the fluid in excess of the first metered volume of fluid, and the fluid actuated closeable valve includes a second absorbent pad configured to absorb the fluid in excess of the second metered volume of fluid.

2. The integrated fluidic device of claim 1, wherein the first overspill chamber is in fluid communication with the metering conduit via a first overspill conduit, and the first overspill conduit is offset from the metering conduit.

3. The integrated fluidic device of claim 1, further comprising a blind fluidic conduit configured to split the second metered volume of fluid from the first metered volume of fluid.

4. The integrated fluidic device of claim 1, further comprising an optical sensor device that measures a length of the second metered volume of fluid to validate the volume of the second metered volume of fluid.

5. The integrated fluidic device of claim 1, further comprising:

a capillary stop at an end of the metering conduit opposite the fluid input chamber; and

a mixing chamber in fluid communication with the capillary stop, wherein the mixing chamber is configured to receive the second metered volume of fluid from the metering conduit through the capillary stop.

6. The integrated fluidic device of claim 5, wherein the mixing chamber has a magnetic flea, and an aspect ratio of a length of the magnetic flea to a diameter of the mixing chamber is at least 0.5.

7. The integrated fluidic device of claim 5, further comprising a closeable lid, and the first overspill chamber includes a vent hole, wherein the closeable lid is configured to close over the vent hole to seal the sample fluid input chamber and force at least a portion of the second metered volume of fluid into the mixing chamber.

8. The integrated fluidic device of claim 5, further comprising at least one further fluid input chamber in fluid communication with the metering conduit, wherein the mixing chamber is configured to receive a further fluid from the at least one further fluid input chamber through the metering conduit and the capillary stop.

9. The integrated fluidic device of claim 8, wherein the at least one further fluid chamber includes a pressure displacement mechanism configured to transfer the further fluid from the at least one further fluid chamber into the metering conduit.

10. The integrated fluidic device of claim 9, wherein the at least one further fluid chamber has first and second frangible seals, and the pressure displacement mechanism is a moveable piston;

and

wherein the moveable piston has a needle that is configured to pierce the first and second frangible seals to transfer the further fluid from the at least one further fluid chamber into the metering conduit.

11. The integrated fluidic device of claim 8, wherein the integrated fluidic device has n further fluid input chambers, where n is greater than or equal to two.

12. The integrated fluidic device of claim 1, further comprising:

an output conduit in fluid communication with the mixing chamber; and

a cell counter in fluid communication with the output conduit;

wherein the output conduit is configured to transfer fluid to the cell counter from the mixing chamber, and the cell counter is configured to count cells that are present in the fluid transferred from the mixing chamber.

13. The integrated fluidic device of claim 12, further wherein the cell counter comprises at least one of a white blood cell counter or a hemoglobin measurement chamber.