Reaction vessel
First Claim
1. A reaction vessel for real time PCR comprising:
- a radially symmetrical reaction vessel base comprising a closed bottom end; and
a plug comprising a handling feature, the handling feature configured to receive a pipette mandrel, and comprising an essentially cylindrical enclosure, the handling feature further comprising;
an inner surface;
an outer surface; and
a longitudinal groove in a direction corresponding with the axial direction in which the plug receives the pipette mandrel;
wherein the reaction vessel base comprises an upper cylindrical portion comprising a latching feature that projects centrally towards the center of the reaction vessel base and engages the plug on insertion where the latching feature contracts centrally on seating of the plug in the reaction vessel, the latching feature irreversibly securing the plug to the radially symmetrical reaction vessel base.
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Accused Products
Abstract
Systems and methods for processing and analyzing samples are disclosed. The system may process samples, such as biological fluids, using assay cartridges which can be processed at different processing locations. In some cases, the system can be used for PCR processing. The different processing locations may include a preparation location where samples can be prepared and an analysis location where samples can be analyzed. To assist with the preparation of samples, the system may also include a number of processing stations which may include processing lanes. During the analysis of samples, in some cases, thermal cycler modules and an appropriate optical detection system can be used to detect the presence or absence of certain nucleic acid sequences in the samples. The system can be used to accurately and rapidly process samples.
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Citations
22 Claims
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1. A reaction vessel for real time PCR comprising:
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a radially symmetrical reaction vessel base comprising a closed bottom end; and a plug comprising a handling feature, the handling feature configured to receive a pipette mandrel, and comprising an essentially cylindrical enclosure, the handling feature further comprising; an inner surface; an outer surface; and a longitudinal groove in a direction corresponding with the axial direction in which the plug receives the pipette mandrel; wherein the reaction vessel base comprises an upper cylindrical portion comprising a latching feature that projects centrally towards the center of the reaction vessel base and engages the plug on insertion where the latching feature contracts centrally on seating of the plug in the reaction vessel, the latching feature irreversibly securing the plug to the radially symmetrical reaction vessel base. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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Specification