Methods, systems, and devices relating to open microfluidic channels

• US 9,289,763 B2
• Filed: 07/23/2013
• Issued: 03/22/2016
• Est. Priority Date: 07/23/2012
• Status: Active Grant

• Current Assignee: Tasso, Inc.
• Original Assignee: Tasso, Inc.
• Inventors: Berthier, Erwin, Casavant, Ben, Moga, Ben
• Primary Examiner(s): Sines, Brian J
• Application Number:
  US13/949,108
• Publication Number:
  US 20140038306A1
• Time in Patent Office: 973 Days
• Field of Search: 422/502, 422/503, 422/504
• US Class Current: 1/1
• CPC Class Codes: A61B 5/1411 by percutaneous method, e.g. by lancet
• CPC Class Codes: A61B 5/150022 for capillary blood or interstitial fluid
• CPC Class Codes: A61B 5/150061 Means for enhancing collection
• CPC Class Codes: A61B 5/150221 Valves
• CPC Class Codes: A61B 5/150343 Collection vessels for collecting blood samples from the skin surface, e.g. test tubes, cuvettes sampling from within body cavities A61B5/150015, A61B5/150045
• CPC Class Codes: A61B 5/150419 comprising means for capillary action
• CPC Class Codes: A61B 5/150969 Low-profile devices which resemble patches or plasters, e.g. also allowing collection of blood samples for testing
• CPC Class Codes: A61B 5/150984 Microneedles or microblades
• CPC Class Codes: A61B 5/15105 Purely manual piercing, i.e. the user pierces the skin without the assistance of any driving means or driving devices
• CPC Class Codes: A61B 5/15146 Devices loaded with multiple lancets simultaneously, e.g. for serial firing without reloading, for example by use of stocking means. multiple simultaneous cutting or piercing A61B5/150977
• CPC Class Codes: B01L 2200/0621 Control of the sequence of chambers filled or emptied
• CPC Class Codes: B01L 2200/0684 Venting, avoiding backpressure, avoid gas bubbles
• CPC Class Codes: B01L 2300/0672 Integrated piercing tool
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0861 Configuration of multiple channels and/or chambers in a single devices
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/161 Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0688 surface tension valves, capillary stop, capillary break
• CPC Class Codes: B01L 2400/086 using baffles or other fixed flow obstructions
• CPC Class Codes: B01L 3/5027 : by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502723 : characterised by venting arrangements
• CPC Class Codes: B01L 3/50273 : characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502738 : characterised by integrated valves microvalves F16K99/0001

Abstract

The various embodiments described herein relate to fabricating and using open microfluidic networks according to methods, systems, and devices that can be used in applications ranging from home-testing, diagnosis, and research laboratories. Open microfluidic networks allow the input, handling, and extraction of fluids or components of the fluid into or out of the open microfluidic network. Fluids can be inserted into an open microfluidic channel by using open sections of the open microfluidic network. Passive valves can be created in the microfluidic network, allowing the creation of logic circuits and conditional flow and volume valves. The fluid can be presented via the microfluidic network to diagnostic and analysis components. Fluids and components of the fluid can be extracted from the open microfluidic network via functional open sections that are easily interfaced with other microfluidic networks or common laboratory tools.

Claims

1. An open-channel microfluidic device comprising a first open microscaled channel configured to allow the flow of a fluid, wherein at least one portion of the channel comprises a first cross-section comprising:

a. at least one wetted surface defining a wetted perimeter length, wherein the wetted surface contacts a fluid flowing through the channel at a contact angle; and

b. at least one free surface comprising an open air-liquid interface defining a free perimeter length;

wherein the ratio of the free perimeter length to the wetted perimeter length is less than the cosine of the contact angle, thereby enabling spontaneous capillary flow.

2. The device of claim 1, further comprising a top portion and a bottom portion, wherein the first cross-section comprises a rectangular or trapezoidal shape, and further comprising a first free surface defined by the top portion of the first cross-section and a second free surface defined by the bottom portion of the first cross-section.

3. The device of claim 2, wherein the second free surface is defined in a bottom portion of the device such that the second free surface can be brought into contact with a volume of fluid pooling on a surface, thereby causing capture of at least a portion of the volume and flow of the volume into the groove.

4. The device of claim 1, wherein the wetted surface comprises a hydrophilic material, the open air-liquid interface is configured to provide access for the removal of a fluid sample from the channel or any component of that fluid sample and receive a second capillary channel, thereby allowing the fluid flow into a second fluidic network.

5. The device of claim 2, wherein the first or second open air-liquid interface is configured to receive a second capillary channel, thereby allowing the fluid flow into a second fluidic network.

6. The device of claim 1, further comprising an aperture defined in the channel, wherein the aperture provides fluid access to an external environment.

7. The device of claim 1, further comprising:

a. a second channel cross-section defining a second free perimeter and second wetted surface, wherein the at least one of the free perimeter or a second wetted perimeter, is greater in size in comparison to the first cross-section free perimeter or wetted perimeter; and

b. a transitional channel region between the first and second cross-sections configured such that the channel progressively narrows between a greater perimeter cross-section and a smaller perimeter cross-section such that the flow is only enabled in the direction of the first cross-section unless additional fluid is added to the second cross-section.

8. The device of claim 1, wherein the first channel is in fluid communication with a common area, wherein at least one additional channel is also in fluid communication with the a common area, thereby allowing device filling independent of synchronized fluid additions.

9. The device of claim 2, further comprising additional protrusive features disposed within the first channel so as to decrease the ratio of the free perimeter and the wetted perimeter, thereby allowing for the capillary filling over a second channel having a larger free perimeter and the collection of larger volumes of fluid.

10. The device of claim 1, wherein the first channel further comprises a material disposed against a wetted surface of the first channel, wherein the material is configured to be incorporated into a flowing fluid.

19. The device of claim 1, wherein the open-channels are configured to prevent the formation of air bubbles.

20. The device of claim 19, wherein the open-channel network allows for the integration of fluids from multiple paths and the resulting configuration further removes air bubbles using a specific geometry of the free surface.

21. The device of claim 1, further comprising a second open microscaled channel in fluidic communication with the first microscaled channel and configured to allow the flow of a fluid, wherein at least one portion of the second channel comprises a second cross-section comprising:

a. at least one wetted surface defining a wetted perimeter length, wherein the wetted surface contacts a fluid flowing through the channel at a contact angle; and

b. at least one free surface comprising an open air-liquid interface defining a free perimeter length; and

wherein the ratio of the second free perimeter length to the second wetted perimeter length is less than the cosine of the contact angle, thereby enabling spontaneous capillary flow.

22. The device of claim 21, wherein the first microscale channel is a longitudinal channel oriented in a first direction and the second microscale channel is a longitudinal channel oriented in a second direction such that the flow of a fluid between the first channel and second channel incorporates a change in direction.

11. A method for using an open microscale channel, the method comprising:

a. providing fluid to or removing fluid from a first open microscale channel, the first channel comprising a first cross-section comprising;

a. a wetted surface, wherein the wetted surface contacts fluid flowing through the channel which defines a wetted perimeter; and

b. a free interface comprising an open air-liquid interface which defines a free perimeter;

wherein the fluid has a contact angle on the wetted surface, the contact angle further comprising a cosine; and

further wherein the ratio of a cross-sectional length of the free perimeter to a cross-sectional length of the wetted perimeter is less than the cosine of the fluid contact angle, thereby enabling spontaneous capillary flow.

12. The method of claim 11, wherein the providing fluid to the first microscale channel comprises contacting the first channel with a fluid pooling on a surface, thereby drawing the fluid into the first channel.

13. The method of claim 12, wherein the fluid is blood and the surface is the surface of the skin.

14. The method of claim 11, further comprising second open microscale channel, the second channel comprising a second cross-section comprising:

a. a wetted surface, wherein the wetted surface defines a wetted perimeter and contacts fluid flowing through the channel at a contact angle; and

b. a free interface comprising an open air-liquid interface which defines a free perimeter; and

wherein the second channel is in fluidic communication with the first channel so as to enable fluid flow between the channels, further wherein the second channel has a second cross-section with a ratio of a cross-sectional length of a free interface to a cross-sectional length of a wetted surface that is smaller than the ratio of the first cross-section.

15. The method of claim 11, wherein the removing the fluid from the first microscale channel comprises removing a substance from the fluid through an aperture defined in the channel.

16. The method of claim 15, wherein the removing the substance from the fluid comprises removing magnetic beads by applying a magnetic force at the window.

17. The method of claim 16, wherein the removing the magnetic beads comprises trapping the beads on a solid surface by placing the solid surface in substantially proximity with or in contact with the surface of the liquid at the window.

18. The method of claim 15, wherein removing the substance from the fluid comprises removing particles by trapping the particles on a material placed in contact with the fluid interface at the window, wherein the material comprises compounds configured to bind the particles.

23. An open-channel microfluidic device comprising a first open microscaled channel configured to allow the flow of a fluid, wherein the channel comprises a generally quadrilateral cross section, comprising:

a. a top portion comprising a first free surface defined by the top portion defining a first free perimeter;

b. a bottom portion comprising a second free surface defined by the bottom portion defining a second free perimeter;

c. a first wetted surface defining a first wetted perimeter, wherein the first wetted surface contacts a fluid flowing through the channel at a first contact angle; and

ii. a second wetted surface defining a second wetted perimeter, wherein the first wetted surface contacts a fluid flowing through the channel at a second contact angle;

wherein the ratio of the first and second free perimeters to the first and second wetted perimeters are less than the cosine of the first and second contact angles, respectively, thereby enabling spontaneous capillary flow through the channel.

24. The device of claim 23, wherein the open-channel microfluidic device is configured to promote three-dimensional fluid flow by allowing fluidic travel in multiple planes relative to the surface of the device.