Tissue engineered blood vessel

• US 9,290,742 B2
• Filed: 03/13/2013
• Issued: 03/22/2016
• Est. Priority Date: 04/30/2008
• Status: Active Grant

• Current Assignee: Cordis Corporation (Cardinal Health, Inc.)
• Original Assignee: Cordis Corporation (Cardinal Health, Inc.)
• Inventors: Cooper, Kevin, Chun, Iksoo, Colter, David C., Dhanaraj, Sridevi, Gosiewska, Anna, Seyda, Agnieszka, Fang, Carrie H., Yang, Chunlin
• Primary Examiner(s): Ghafoorian, Reza
• Application Number:
  US13/799,520
• Publication Number:
  US 20130196431A1
• Time in Patent Office: 1,105 Days
• US Class Current: 1/1
• CPC Class Codes: A61L 27/18 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: A61L 27/3604 characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
• CPC Class Codes: A61L 27/38 containing added animal cells organs or tissue containing native cells A61L27/36
• CPC Class Codes: A61L 27/507 for artificial blood vessels apparatus for applying cells on a blood vessel prosthesis A61F2/062
• CPC Class Codes: C08L 67/04 Polyesters derived from hydroxycarboxylic acids, e.g. lactones C08L67/06 takes precedence
• CPC Class Codes: C12M 21/08 for producing artificial tissue or for ex-vivo cultivation of tissue prostheses A61F2/00, grafts A61L27/00
• CPC Class Codes: C12M 25/14 Scaffolds; Matrices in general C12N5/0068
• CPC Class Codes: C12N 5/0691 Vascular smooth muscle cells; 3D culture thereof, e.g. models of blood vessels

Abstract

Compositions and methods of using tissue engineered blood vessels to repair and regenerate blood vessels of patients with vascular disease are disclosed.

Claims

1. A method of making a tissue engineered blood vessel comprising the steps of:

a. Providing a scaffold comprising a solution of poly(p-dioxanone) and 1,1,1,3,3,3-hexafluoro-2-propanol solvent formed as an electrospun tubular scaffold of randomly oriented fibers;

b. Cutting the electrospun tubular scaffold to form a scaffold sheet;

c. Seeding said scaffold sheet with cells;

d. Rolling said seeded sheet into a tube; and

e. Culturing the tube in a bioreactor.