Putative cytokinin receptor and methods for use thereof

• US 9,290,777 B2
• Filed: 02/05/2008
• Issued: 03/22/2016
• Est. Priority Date: 02/05/2007
• Status: Expired due to Fees

• Current Assignee: National University of Singapore
• Original Assignee: National University of Singapore
• Inventors: Kumar, Prakash Pallathadka, Godge, Mandar Radhakisan
• Primary Examiner(s): Zhou, Shubo (Joe)
• Assistant Examiner(s): KOVALENKO, MYKOLA V
• Application Number:
  US12/525,968
• Publication Number:
  US 20110023187A1
• Time in Patent Office: 2,968 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: C07K 14/415 from plants
• CPC Class Codes: C12N 15/8261 with agronomic (input) traits, e.g. crop yield
• CPC Class Codes: C12N 15/8295 Cytokinins
• CPC Class Codes: Y02A 40/146 Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

A method of modulating the expression of at least one trait in a plant, the method comprising the step of modulating the expression of at least one polypeptide by the plant, wherein the polypeptide is selected from the group consisting of: i) a polypeptide which comprises the amino acid sequence according to SEQ ID NO:1; ii) a polypeptide which comprises an amino acid sequence which is selected from the group consisting of any one or more of from amino acid 1 to amino acid 7, from amino acid 9 to amino acid 230, from amino acid 1 to amino acid 58, from amino acid 77 to amino acid 485, from amino acid 59 to amino acid 76, from amino acid 150 to amino acid 191, from amino acid 231 to amino acid 405, and from amino acid 406 to amino acid 438 of SEQ ID NO: 1, or at equivalent positions in a homologue thereof, and which is capable of modulating cytokinin signaling in the plant; iii) a polypeptide which comprises an amino acid sequence with at least 70% sequence identity to the amino acid sequence according to SEQ ID NO:1 and which is capable of modulating cytokinin signaling in the plant; and iv) a polypeptide which consists of an amino acid sequence according to SEQ ID NO:1, wherein the modulation of the expression of the polypeptide modulates the expression of at least one trait in the plant.

Claims

1. A method of modulating the expression of at least one trait in a rice plant, the method comprising:

decreasing the expression of a polypeptide in a rice plant, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO;

5, wherein decreasing the expression of the polypeptide comprises

introducing into one or more cells of the rice plant a polynucleotide which decreases the expression of the polypeptide to produce a transformed rice plant, wherein the introduced polynucleotide is selected from the group consisting of;

i) an antisense polynucleotide which comprises the nucleic acid sequence of SEQ ID NO;

15, and

ii) an antisense polynucleotide which consists of the nucleic acid sequence of SEQ ID NO;

15; and

selecting for decreased expression of the polypeptide and for modulated expression of at least one trait in the rice plant,

wherein the modulated expression of at least one trait in the rice plant is selected from the group consisting of increased number of panicles, increased seed yield, increased plant biomass, increased number of tillers, and increased leaf area, relative to the expression of the same trait in a non-transformed rice plant.

2. The method according to claim 1, wherein the increased number of panicles is up to 5-fold, the increased number of seeds per plant is up to 2.7-fold, the increase in plant biomass is up to 3.6-fold, the increased number of tillers is up to 4-fold, and the increased leaf area is up to 1.25 fold, relative to a non-transformed rice plant.

3. A method of producing a transgenic rice plant with at least one modulated trait comprising:

transforming a rice plant, rice plant part or rice plant cell with a polynucleotide comprising

i) an antisense polynucleotide which comprises the nucleic acid sequence of SEQ ID NO;

15, or

ii) an antisense polynucleotide which consists of the nucleic acid sequence of SEQ ID NO;

15;

growing the transformed rice plant, rice plant part or rice plant cell to produce a transgenic rice plant; and

selecting the transgenic rice plant for at least one trait selected from the group consisting of increased plant biomass, increased number of panicles, increased leaf area, increased seed yield, and increased tiller number, relative to the same trait in a non-transformed rice plant.

4. The method according to claim 3, wherein the growing comprises culturing the transformed rice plant, rice plant part or rice plant cell under conditions which permit growth of the transformed rice plant, rice plant part or rice plant cell.

5. The method according to claim 3, wherein the rice plant part is selected from the group consisting of root, stem, leaf, bud, flower, shoot, seed and branch.

6. The method of claim 3, wherein said transgenic plant is capable of producing fertile plants.

8. The method of claim 3, wherein the antisense polynucleotide decreases expression of a rice polypeptide comprising the amino acid of SEQ ID NO:

5.

7. A method of producing a rice plant with increased biomass, the method comprising

transforming a rice plant with an isolated polynucleotide selected from the group consisting of:

i) an antisense polynucleotide comprising the nucleic acid sequence of SEQ ID NO;

15 and

ii) an antisense polynucleotide which consists of the nucleic acid sequence of SEQ ID NO;

15; and

selecting the transformed plant for the expression of said polynucleotide and increased biomass.