Alpha-neoagarobiose hydrolase and method for obtaining a monosaccharide using same

• US 9,297,000 B2
• Filed: 09/14/2011
• Issued: 03/29/2016
• Est. Priority Date: 03/27/2009
• Status: Active Grant

• Current Assignee: Korea University Research and Business Foundation
• Original Assignee: Korea University Research and Business Foundation
• Inventors: Choi, In Geol, Lee, Sae Young, Kim, Kyoung Heon
• Primary Examiner(s): HUTSON, RICHARD G
• Application Number:
  US13/232,535
• Publication Number:
  US 20130171699A1
• Time in Patent Office: 1,658 Days
• Field of Search: 435/195, 435/200
• US Class Current: 1/1
• CPC Class Codes: C12N 9/24 acting on glycosyl compounds (3.2)
• CPC Class Codes: C12N 9/2402 hydrolysing O- and S- glycosyl compounds (3.2.1)
• CPC Class Codes: C12P 19/14 produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase , e.g. by cellulase, hemicellulase

Abstract

Provided are novel α-neoagarobiose hydrolase and a method for obtaining a monosaccharide using the same.

Claims

1. A method for preparing galactose 3,6-anhydro-L-galactose (AHG), comprising steps of:

obtaining an α

-neoagarobiose hydrolase from a culture of host cells transformed with a recombinant vector, wherein the recombinant vector comprises an isolated nucleic acid molecule encoding a protein comprising the amino acid sequence set forth in SEQ ID NO;

2;

combining an α

-neoagarobiose, a buffer and the α

-neoagarobiose hydrolase to form a reaction solution;

heating the reaction solution at a temperature effective to cause a reaction between the α

neoagarobiose and the α

-neoagarobiose hydrolase and

obtaining galactose or AHG from the degradation product.