Activity of Fe—S cluster requiring proteins
First Claim
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1. A recombinant yeast host cell comprising:
- (a) at least one heterologous polynucleotide encoding a dihydroxy-acid dehydratase enzyme (DHAD), wherein the dihydroxy-acid dehydratase enzyme has an amino acid sequence with at least 95% identity to SEQ ID NO;
168; and
(b) at least one deletion in an endogenous gene encoding a polypeptide affecting Fe—
S cluster biosynthesis, wherein the gene encoding the polypeptide affecting Fe—
S cluster biosynthesis is FRA2.
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Abstract
The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe—S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe—S cluster biosynthesis pathway in a host cell.
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Citations
13 Claims
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1. A recombinant yeast host cell comprising:
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(a) at least one heterologous polynucleotide encoding a dihydroxy-acid dehydratase enzyme (DHAD), wherein the dihydroxy-acid dehydratase enzyme has an amino acid sequence with at least 95% identity to SEQ ID NO;
168; and(b) at least one deletion in an endogenous gene encoding a polypeptide affecting Fe—
S cluster biosynthesis, wherein the gene encoding the polypeptide affecting Fe—
S cluster biosynthesis is FRA2. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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Specification