Use of perturbants to facilitate incorporation and recovery of taggants from polymerized coatings

• US 9,297,032 B2
• Filed: 10/10/2012
• Issued: 03/29/2016
• Est. Priority Date: 10/10/2012
• Status: Active Grant

• Current Assignee: APDN (B.V.I.) Inc. (Applied DNA Sciences Incorporated)
• Original Assignee: APDN (B.V.I.) Inc. (Applied DNA Sciences Incorporated)
• Inventors: Jung, Lawrence, Liang, MingHwa Benjamin, Hayward, James A.
• Primary Examiner(s): WOOLWINE, SAMUEL C
• Application Number:
  US13/648,594
• Publication Number:
  US 20140099643A1
• Time in Patent Office: 1,266 Days
• US Class Current: 1/1
• CPC Class Codes: C12Q 1/68 involving nucleic acids
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 2563/185 Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals

Abstract

The invention provides methods for increasing the recoverability of taggants from an object. The methods include the steps of incorporating a taggant into a solution; mixing the solution including the taggant with a perturbant to form a first perturbant taggant solution; mixing the first perturbant taggant solution with a polymer to form a second perturbant taggant polymer solution; and applying the second perturbant taggant polymer solution to at least a portion of the object to form a taggant-coated object. Methods for authentication of a taggant marked object are also provided.

Claims

1. A method for authenticating an object, comprising:

providing an object comprising a coating comprising a taggant, a perturbant and a polymer;

wherein the taggant comprises a nucleic acid;

wherein the perturbant comprises one or both of a hydroxypropyl starch and a pyrrolidone compound;

wherein the polymer comprises one or more of polyurethane, a shellac, a lacquer, an acrylic compound and an epoxy-based compound;

recovering the nucleic acid of the taggant from the object, wherein the perturbant increases recoverability of the nucleic acid taggant; and

verifying the authenticity of the object by identifying the nucleic acid of the taggant.

2. The method of claim 1, wherein the taggant comprises DNA and one or more of an upconverting phosphor (UCP), a UV fluorophore, a ceramic IR marker, an amino acid, a protein, a pheromone, a scent, a trace element and a rare earth element.

3. The method of claim 2, wherein the taggant consists essentially of DNA and either an upconverting phosphor (UCP), or a UV fluorophore.

4. The method of claim 2, wherein the DNA recovered from the object is identified using a method comprising PCR or FISH.

5. The method of claim 4, wherein the DNA is authenticated in a PCR reaction using primers specific to the DNA to produce specific amplicons, and identifying of the number of the specific amplicons and the length of each of the specific amplicons amplified in the polymerase chain reaction.

6. The method of claim 1, wherein an amount of the perturbant is in a range from about 0.1 to about 30% w/w of the polymer.

7. The method of claim 6, wherein an amount of the perturbant is in a range from about 1% to about 20% w/w of the polymer.

8. The method of claim 7, wherein an amount of the perturbant is in a range from about 1% to about 10% w/w of the polymer.

9. The method of claim 1, wherein the taggant comprises DNA.

10. A method for authenticating an object, comprising:

providing an object comprising one or more taggants comprising a nucleic acid, a perturbant and a polymer;

wherein the perturbant comprises one or one or both of a hydroxypropyl starch and a pyrrolidone compound; and

wherein the polymer is selected from the group consisting of at least one of polyurethane (PU), polystyrene (PS), polypropylene (PP), polyvinyl chloride (PVC), polyvinylacetate (PVA), polyester (PES), polyethylene terephthalate (PET), polyethylene (PE) and polyvinylidene chloride (PVDC);

recovering the one or more taggants from the object, wherein the perturbant increases recoverability of the nucleic acid taggant; and

verifying the authenticity of the object by identifying one or more of the taggants.

11. The method of claim 10, wherein the taggant comprises DNA and one or more of, an upconverting phosphor (UCP), a UV fluorophore, a ceramic IR marker, an amino acid, a protein, a pheromone, a scent, a trace element and a rare earth element.

12. The method of claim 11, wherein the DNA recovered from the object is authenticated using a method comprising PCR or FISH.

13. The method according to claim 12, wherein the DNA taggant is authenticated in a PCR reaction using primers specific to the DNA taggant to produce specific amplicons, and identifying of the number of the specific amplicons and the length of each of the specific amplicons amplified in the polymerase chain reaction.

14. The method of claim 10, wherein an amount of the perturbant is in a range from about 0.1 to about 30% w/w of the polymer.

15. The method of claim 14, wherein an amount of the perturbant is in a range from about 1% to about 20% w/w of the polymer.

16. The method of claim 15, wherein an amount of the perturbant is in a range from about 1% to about 10% w/w of the polymer.