Chemically cleavable 3′-O-allyl-dNTP-allyl-fluorophore fluorescent nucleotide analogues and related methods
First Claim
Patent Images
1. A nucleotide analogue selected from the group consisting of
1 Assignment
0 Petitions
Accused Products
Abstract
This invention provides a nucleotide analog comprising (i) a base selected from the group consisting of adenine, guanine, cytosine, thymine and uracil, (ii) a deoxyribose, (iii) an allyl moiety bound to the 3′-oxygen of the deoxyribose and (iv) a fluorophore bound to the base via an allyl linker, and methods of nucleic acid sequencing employing the nucleotide analog.
-
Citations
19 Claims
- 1. A nucleotide analogue selected from the group consisting of
- 4. A method for making a nucleotide analogue wherein the nucleotide analogue is selected from the group consisting of
-
12. A method for determining the sequence of a DNA comprising performing the following steps for each residue of the DNA to be sequenced:
-
(a) contacting the DNA with a DNA polymerase in the presence of (i) a primer and (ii) four fluorescent nucleotide analogues under conditions permitting the DNA polymerase to catalyze DNA synthesis,
wherein the nucleotide analogues are - View Dependent Claims (13, 14, 15, 16, 17, 18)
-
Specification