Ion selective electrode module for clinical diagnostics

• US 9,297,797 B2
• Filed: 06/30/2010
• Issued: 03/29/2016
• Est. Priority Date: 07/01/2009
• Status: Expired due to Fees

• Current Assignee: Beckman Coulter Incorporated (Danaher Corporation)
• Original Assignee: Beckman Coulter Incorporated (Danaher Corporation)
• Inventors: Situ, Richard Lou, Xie, Youqin E.
• Primary Examiner(s): Van, Luan
• Assistant Examiner(s): RUFO, LOUIS J
• Application Number:
  US12/827,975
• Publication Number:
  US 20110000796A1
• Time in Patent Office: 2,099 Days
• Field of Search: 204/267, 204/232, 205/779, 205/789.5, 205/792, 205/789
• US Class Current: 1/1
• CPC Class Codes: G01N 27/333 Ion-selective electrodes or membranes glass electrodes G01N27/36
• CPC Class Codes: G01N 33/4915 using flow cells flow cytometry G01N15/14

Abstract

A method of determining an electrolyte in a sample including adding the sample to an electrolyte module, the electrolyte module including a dilution cup, a flow cell, and a pump, the flow cell having a flow channel with a first end and a second end, the first end fluidically coupled to the dilution cup, and the second end fluidically coupled to the pump; combining the sample with a diluent in the dilution cup to produce a diluted sample; operating the pump to aspirate the diluted sample into the flow cell; measuring the electrolyte in the diluted sample in the flow cell; and reversing the pump to dispense fluid through the second end to displace the diluted sample from the flow cell back into the dilution cup.

Claims

1. A method of determining an electrolyte in a sample, the method comprising:

adding the sample to an electrolyte module, the electrolyte module including a single, integrated body and a pump, the single, integrated body including a dilution cup and a flow cell, the dilution cup having a hemispherical bottom and being formed within the single, integrated body, the flow cell having a flow channel with a first end and a second end, the first end fluidically coupled to the dilution cup, and the second end fluidically coupled to the pump;

combining the sample with a diluent in the dilution cup to produce a diluted sample;

operating the pump to aspirate the diluted sample into the flow cell through the first end;

measuring the electrolyte in the diluted sample in the flow cell; and

reversing the pump to dispense a reference solution through the second end to displace the diluted sample from the flow cell back into the dilution cup and to fill the flow cell with the reference solution,

wherein the flow cell further includes a side channel having a junction end and a free end, the junction end fluidically coupled to the flow channel at an intersection in between the first end and the second end,

wherein a reference solution is added to the flow cell through either of two paths, wherein one of the two paths includes the side channel and the intersection, and wherein the other of the two paths includes the second end and the intersection; and

wherein adding the reference solution to the flow cell through either of the two paths includes actuating a bidirectional valve to direct the reference solution through one or the other of the two paths.

2. The method of claim 1 further comprising draining the displaced diluted sample from the dilution cup.

3. The method of claim 1 wherein operating the pump includes removing flow channel contents through the second end, thereby forming a liquid junction at the intersection.

4. The method of claim 1 further comprising configuring an isolation valve on the electrolyte module between the dilution cup and the flow cell, and wherein operating the pump includes opening the isolation valve.

5. The method of claim 4 wherein measuring the electrolyte includes closing the isolation valve.

6. The method of claim 1 wherein combining the sample with a diluent includes injecting a first diluent aliquot, adding the sample, and injecting a second diluent aliquot.

7. The method of claim 6 wherein the volume of the second diluent aliquot is greater than the sum of the volumes of the first diluent aliquot and the sample.

8. The method of claim 7 wherein the injection rate of the second diluent aliquot is about 500 microliters per second.

9. The method of claim 8 further comprising rinsing the dilution cup.

14. The method of claim 1, wherein the dilution cup has an open top.

15. The method of claim 1, further comprising:

cleaning a probe in the dilution cup.

16. The method of claim 1, wherein cleaning the probe comprises:

substantially filling the dilution cup with clean wash fluid;

inserting the probe into the clean wash fluid in the dilution cup to rinse the outside of the probe;

removing the probe from the wash fluid; and

flushing the wash fluid from the dilution cup.

10. A method of determining an electrolyte in a sample, the method comprising:

providing an electrolyte module including a single, integrated body having a dilution cup and a flow cell, the dilution cup and the flow cell formed within the integrated body, the dilution cup having an open top, a hemispherical bottom, and a diluent conduit fluidically connected to the dilution cup, the flow cell disposed between the dilution cup and a reference pump, wherein the reference pump is configured to dispense reference solution by delivering the reference solution from a reference container to the flow cell through an end of the flow cell opposite the dilution cup;

forming a diluted sample in the dilution cup by injecting a first diluent aliquot, adding the sample, and injecting a second diluent aliquot; and

measuring the electrolyte in the diluted sample, wherein the volume of the second diluent aliquot is greater than the sum of the volumes of the first diluent aliquot and the sample.

11. The method of claim 10 further comprising connecting the diluent conduit to the dilution cup within the hemispherical bottom so that the diluent conduit extends parallel to the open top, and injecting the second diluent aliquot into the dilution cup through the diluent conduit.

12. The method of claim 11 wherein the injection rate of the second diluent aliquot is about 500 microliters per second.

13. The method of claim 10 wherein the electrolyte module further includes a transfer probe movably disposed with respect to the dilution cup and aligned with the open top, and wherein the sample enters the dilution cup through the transfer probe.