Method of preparing autologous cells and method of use for therapy
First Claim
1. A method of treating a patient, said patient having tissue in or near a target area of a disease, with an autologous therapeutic cell population expanded in autologous medium comprising:
- selecting a patient having a disease selected from the group consisting of;
heart failure, myocardial ischemia, and Crohn'"'"'s disease;
harvesting blood from the selected patient in two visits separated between one and three weeks;
harvesting bone marrow cells from the selected patient;
forming a culture medium from said blood that contains no animal serum and comprises 10% autologous serum obtained from the patient;
expanding a population of mesenchymal stem cells from the bone marrow cells in the culture medium; and
injecting the expanded mesenchymal stem cells into the tissue of the selected patient in or near the target area of the disease;
thereby ameliorating the effect of the disease.
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Abstract
A method for expanding mesenchymal cells derived from autologous bone marrow in autologous culture medium which can be used in a clinical setting, and a business method for performing such expansions in the future as a service for patients. A method for expanding mesenchymal cells derived from autologous bone marrow in autologous culture medium including a diagnostic kit for the autologous cell therapy to determine whether a patient will respond to the autologous cell therapy for treatment of a disease, in which said kit comprising a system for detecting gene and protein expression comprising at least two isolated DNA molecules wherein each isolated DNA molecule detects expression of a gene that is differentially expressed in the tissue of the patient that is intended to be the source of the autologous cell therapy.
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Citations
10 Claims
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1. A method of treating a patient, said patient having tissue in or near a target area of a disease, with an autologous therapeutic cell population expanded in autologous medium comprising:
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selecting a patient having a disease selected from the group consisting of;
heart failure, myocardial ischemia, and Crohn'"'"'s disease;harvesting blood from the selected patient in two visits separated between one and three weeks; harvesting bone marrow cells from the selected patient; forming a culture medium from said blood that contains no animal serum and comprises 10% autologous serum obtained from the patient; expanding a population of mesenchymal stem cells from the bone marrow cells in the culture medium; and
injecting the expanded mesenchymal stem cells into the tissue of the selected patient in or near the target area of the disease;thereby ameliorating the effect of the disease. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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Specification