Methods and products for tissue repair

• US 9,308,242 B2
• Filed: 04/15/2013
• Issued: 04/12/2016
• Est. Priority Date: 09/28/2006
• Status: Active Grant

• Current Assignee: Children's Medical Center Corporation
• Original Assignee: Children's Medical Center Corporation
• Inventors: Murray, Martha M.
• Primary Examiner(s): Gudibande, Satyanarayana R
• Application Number:
  US13/862,554
• Publication Number:
  US 20130273017A1
• Time in Patent Office: 1,093 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: A61F 2/0811 Fixation devices for tendons or ligaments
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 31/401 Proline; Derivatives thereof, e.g. captopril
• CPC Class Codes: A61K 35/17 Lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes when activated by a specific antigen A61K39/00
• CPC Class Codes: A61K 35/19 Platelets; Megacaryocytes
• CPC Class Codes: A61K 38/39 Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
• CPC Class Codes: A61K 45/06 Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• CPC Class Codes: A61L 2300/252 Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
• CPC Class Codes: A61L 2300/406 Antibiotics
• CPC Class Codes: A61L 2300/432 Inhibitors, antagonists A61L2300/402 - A61L2300/43 take precedence
• CPC Class Codes: A61L 2300/602 Type of release, e.g. controlled, sustained, slow
• CPC Class Codes: A61L 2300/64 Animal cells
• CPC Class Codes: A61L 2400/06 Flowable or injectable implant compositions
• CPC Class Codes: A61L 2430/10 for reconstruction of tendons or ligaments
• CPC Class Codes: A61L 27/24 Collagen
• CPC Class Codes: A61L 27/3616 Blood, e.g. platelet-rich plasma
• CPC Class Codes: A61L 27/3662 Ligaments, tendons
• CPC Class Codes: A61L 27/50 Materials characterised by their function or physical properties , e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
• CPC Class Codes: A61L 27/54 Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: A61L 27/56 Porous materials, e.g. foams or sponges
• CPC Class Codes: A61L 27/58 : Materials at least partially resorbable by the body
• CPC Class Codes: A61P 19/02 : for joint disorders, e.g. arthritis, arthrosis

Abstract

Methods and devices for the repair of articular tissue using collagen material are provided. Compositions of collagen material and related kits are also provided.

Claims

1. A method comprising:

contacting the ends of a ruptured articular tissue in a subject in need thereof with a sterile solution of solubilized collagen and white blood cells, and allowing the solution to set to treat the ruptured articular tissue, wherein the sterile solution comprises solubilized collagen in a concentration of greater than 5 mg/ml and less than or equal to 50 mg/ml.

2. The method of claim 1, wherein the white blood cells comprise neutrophils.

3. The method of claim 1, wherein the white blood cells comprise basophils.

4. The method of claim 1, wherein the white blood cells comprise eosinophils.

5. The method of claim 1, wherein the white blood cells comprise monocytes.

6. The method of claim 1, wherein the white blood cells comprise a combination of neutrophils, basophils, eosinophils and monocytes.

7. The method of claim 1, wherein the sterile solution of solubilized collagen and white blood cells are premixed prior to application to the ruptured articular tissue.

8. The method of claim 1, wherein the sterile solution of solubilized collagen has a viscosity of 1,000-200,000 centipoise, a hydroxyproline in a concentration of 0.1-5.0 μ

g/ml, and a neutralizing agent, wherein the solution has an osmolarity of 280-350 mOsm/kg.

9. The method of claim 1, wherein the solution is a liquid or gel.

10. The method of claim 1, wherein the solubilized collagen is present in a concentration of greater than 15 mg/ml and less than or equal to 40 mg/ml.

11. The method of claim 1, wherein the white blood cells are in a concentration of at least 4×

10³ wbc/ml.

12. The method of claim 1, wherein the sterile collagen solution is lyophilized prior to adding the white blood cells.

13. The method of claim 11, wherein the sterile collagen solution is lyophilized prior to adding the white blood cells.