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Methods for producing a paired tag from a nucleic acid sequence and methods of use thereof

  • US 9,309,560 B2
  • Filed: 05/19/2009
  • Issued: 04/12/2016
  • Est. Priority Date: 10/31/2003
  • Status: Active Grant
First Claim
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1. A method for preparing a plurality of nucleic acids, comprising:

  • a) producing a plurality of circular nucleic acids, by intramolecularly ligating a plurality of nucleic acid fragments each having one or more first adaptors joined to the 5′ and

    3′

    ends of a linear nucleic acid fragment;

    b) fragmenting the plurality of circularized nucleic acids by shearing, thereby producing a plurality of linear paired tag fragments containing the one or more first adaptors and a paired tag having the 5′

    end and the 3′

    end of the linear nucleic acid fragment in step (a); and

    c) joining a second adapter to both ends of the plurality of linear paired tag fragments thereby producing a plurality of adaptor-joined paired tag fragments.

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