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Compositions and processes for analyte detection, quantification and amplification

  • US 9,309,563 B2
  • Filed: 07/27/2004
  • Issued: 04/12/2016
  • Est. Priority Date: 06/30/2001
  • Status: Active Grant
First Claim
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1. A process for detecting or quantifying multiple different nucleic acid species of interest in a library, the process comprising the steps of:

  • a) providing;

    (i) an array of fixed or immobilized nucleic acids complementary to said nucleic acid species of interest;

    (ii) a library of nucleic acid analytes isolated or derived from a biological source, which library of nucleic acid analytes may contain the nucleic acid species of interest sought to be detected or quantified, wherein each of said nucleic acid species of interest comprises at least one inherent universal detection target (UDT); and

    (iii) labeled universal detection elements (UDE) which generate a signal directly or indirectly;

    b) hybridizing said library (ii) with said array of nucleic acids (i) to form hybrids if said nucleic acid species of interest are present, thereby binding said nucleic acid species of interest comprising said inherent UDTs to said array;

    c) contacting said inherent UDTs on said nucleic acid species of interest bound to said array with said labeled UDEs to form a complex between said labeled UDEs and said inherent UDTs, wherein said complex is bound to said array, and wherein the sequence of said labeled UDE consists of a sequence that binds to a 3′

    polyA segment, or a consensus sequence when said inherent UDT is a nucleic acid; and

    d) detecting or quantifying said multiple different nucleic acid species of interest by detecting or measuring the amount of signal generated from labeled UDEs complexed to inherent UDTs on each nucleic acid species of interest bound to said array.

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