DDAO compounds as fluorescent reference standards
First Claim
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1. A method comprising:
- (a) contacting a sample which may comprise at least one target polynucleotide with at least one probe and a reference dye to form a mixture, wherein each of the at least one probes comprises a target-specific moiety, a reporter dye and a quencher dye, wherein each target-specific moiety is an oligonucleotide and is specific for a different target and each reporter dye is different from the other reporter dyes and is different from the reference dye, and wherein the reference dye has formula (I);
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Abstract
According to the present teachings, methods and compositions are provided that utilize at least one reference dye of formula (I):
In some embodiments, a method comprises measuring a detection signal of a reporter dye and at least one reference dye of formula (I). In some embodiments, a composition comprises a reference dye of formula (1), a buffer, a selection of nucleotides and a protein.
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16 Claims
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1. A method comprising:
(a) contacting a sample which may comprise at least one target polynucleotide with at least one probe and a reference dye to form a mixture, wherein each of the at least one probes comprises a target-specific moiety, a reporter dye and a quencher dye, wherein each target-specific moiety is an oligonucleotide and is specific for a different target and each reporter dye is different from the other reporter dyes and is different from the reference dye, and wherein the reference dye has formula (I); - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 16)
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14. A method comprising:
(a) contacting a sample which may comprise at least one target polynucleotide with at least one probe and a reference dye to form a mixture, wherein each of the at least one probes comprises a target-specific moiety, a reporter dye and a quencher dye, wherein each target-specific moiety is an oligonucleotide and is specific for a different target and each reporter dye is different from the other reporter dyes and is different from the reference dye, and wherein the reference dye has formula;
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15. A method comprising:
(a) contacting a sample which may comprise at least one target polynucleotide with two to six probes and a reference dye to form a mixture, wherein each of the two to six probes comprises a target-specific moiety, a reporter dye and a quencher dye, wherein each target-specific moiety is an oligonucleotide and is specific for a different target and each reporter dye is different from the other reporter dyes and is different from the reference dye, and wherein the reference dye has formula (I);
Specification