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DDAO compounds as fluorescent reference standards

  • US 9,315,859 B2
  • Filed: 11/04/2013
  • Issued: 04/19/2016
  • Est. Priority Date: 12/29/2010
  • Status: Active Grant
First Claim
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1. A method comprising:

  • (a) contacting a sample which may comprise at least one target polynucleotide with at least one probe and a reference dye to form a mixture, wherein each of the at least one probes comprises a target-specific moiety, a reporter dye and a quencher dye, wherein each target-specific moiety is an oligonucleotide and is specific for a different target and each reporter dye is different from the other reporter dyes and is different from the reference dye, and wherein the reference dye has formula (I);

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