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System and method to correct out of phase errors in DNA sequencing data by use of a recursive algorithm

  • US 9,317,654 B2
  • Filed: 12/18/2012
  • Issued: 04/19/2016
  • Est. Priority Date: 02/16/2006
  • Status: Expired due to Fees
First Claim
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1. A method for recursively correcting an error associated with phasic synchrony of nucleic acid sequence data generated from a population of substantially identical copies of a template molecule, comprising:

  • (a) detecting a plurality of signals generated in response to a plurality of nucleotide species introduced during a sequencing reaction;

    (b) generating an observed value for the signal detected from each of the nucleotide species;

    (c) defining a plurality of positive incorporation values and a plurality of negative incorporation values from the observed values using a carry forward value and an incomplete extension value;

    (d) simultaneously calculating a carry forward perturbation increment and an incomplete extension perturbation increment;

    (e) revising the carry forward value and the incomplete extension value using the carry forward perturbation increment and the incomplete extension perturbation increment;

    (f) re-defining the plurality of positive incorporation values and the plurality of negative incorporation values using the revised carry forward value and the revised incomplete extension value; and

    (g) repeating steps (d)-(f) until convergence of the plurality of positive incorporation values and the plurality of negative incorporation values, thereby generating a corrected nucleic acid sequence read.

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