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DNA-binding proteins and uses thereof

  • US 9,322,005 B2
  • Filed: 10/28/2013
  • Issued: 04/26/2016
  • Est. Priority Date: 05/17/2010
  • Status: Active Grant
First Claim
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1. An isolated, non-naturally occurring DNA-binding polypeptide comprising:

  • two or more TALE-repeat units, the TALE repeat units comprising a repeat variable di-residue (RVD) wherein the RVD binds to a nucleotide in a target DNA sequence;

    an N-cap polypeptide flanking the N-terminal portion of the TALE-repeat units, wherein the N-cap polypeptide comprises a fragment of a full length N-terminus region of a TALE protein, the fragment comprising residues between N+122 and N+137; and

    a C-cap polypeptide flanking the C-terminal portion of the TALE-repeat units, wherein the C-cap polypeptide comprises a fragment of a full length C-terminus region of a TALE protein,wherein the polypeptide binds to DNA.

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