Expression constructs comprising fungal promoters

• US 9,322,027 B2
• Filed: 08/22/2011
• Issued: 04/26/2016
• Est. Priority Date: 08/20/2010
• Status: Expired due to Fees

• Current Assignee: Shell Oil Company (Shell Plc)
• Original Assignee: Shell Oil Company (Shell Plc)
• Inventors: Shock, Jennifer L., Clark, Louis
• Primary Examiner(s): Ault, Addison D
• Application Number:
  US13/214,406
• Publication Number:
  US 20120045793A1
• Time in Patent Office: 1,709 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: C07K 14/37 from fungi
• CPC Class Codes: C12N 15/80 for fungi

Abstract

The present invention provides promoters derived from a filamentous fungus. These promoters have application in the fields of molecular biology, microbiology, fungal genetics and production of biofuels and other products.

Claims

1. An expression construct comprising a recombinant polynucleotide having promoter activity comprising a nucleotide sequence with at least 90% sequence identity to the nucleotide sequence set forth in any of SEQ ID NOs:

1, 17, 25, or 33, operably linked to at least one heterologous DNA sequence encoding at least one protein.

2. The expression construct of claim 1, wherein the protein comprises a signal peptide fused to a secreted protein sequence.

3. The expression construct of claim 2, wherein the signal peptide is not associated with the secreted protein in nature.

4. The expression construct of claim 1, wherein said at least one protein comprises at least one enzyme.

5. The expression construct of claim 4, wherein said at least one enzyme comprises at least one cellulase.

6. The expression construct of claim 5, wherein the at least one cellulase comprises an endoglucanase, a cellobiohydrolase andor a p-glucosidase.

7. The expression construct of claim 4, wherein said at least one enzyme comprises a glucoamylase, protease, alpha amylase, cellulase, hemicellulase, xylanase, esterase, cutinase, phytase, lipase, oxidoreductase, reductase, dehydrogenase, synthase, invertase, laccase, isomerase, pullulanase, phenol oxidizing enzyme, mannanase, mannose, catalase, glucose oxidase, transferase, and/or lyase.

8. The expression construct of claim 4, wherein said at least one enzyme comprises at least one recombinant enzyme.

9. A host cell comprising the expression construct of claim 1.

10. The host cell of claim 9, wherein said cell is a yeast or filamentous fungal cell.

11. The host cell of claim 9, wherein said cell is Myceliophthora thermophile.

12. The host cell of claim 9, wherein said expression cassette is integrated into the genome of said host cell.

13. A method for producing a protein in a host cell, comprising culturing the host cell of claim 9, under conditions such that said protein is produced by said host cell.

14. The method of claim 13, further comprising the step of isolating said protein produced by said host cell.