Methods and compositions for inactivating alpha 1,6 fucosyltransferase (FUT8) gene expression
First Claim
Patent Images
1. A method of inactivating an endogenous cellular FUT8 gene in an isolated cell, the method comprising:
- introducing, into an isolated cell, a first nucleic acid encoding a first polypeptide, wherein the first polypeptide comprises;
(a) a first zinc finger DNA-binding domain, wherein the DNA-binding domain comprises four or five zinc finger recognition regions ordered F1 to F4, F1 to F5 or F1 to F6 from N-terminus to C-terminus, and wherein F1 to F4, F1 to F5 or F1 to F6 comprise amino acid sequences selected from the group consisting of;
1 Assignment
0 Petitions
Accused Products
Abstract
Disclosed herein are methods and compositions for inactivating a FUT8 gene, using fusion proteins comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.
-
Citations
12 Claims
-
1. A method of inactivating an endogenous cellular FUT8 gene in an isolated cell, the method comprising:
-
introducing, into an isolated cell, a first nucleic acid encoding a first polypeptide, wherein the first polypeptide comprises; (a) a first zinc finger DNA-binding domain, wherein the DNA-binding domain comprises four or five zinc finger recognition regions ordered F1 to F4, F1 to F5 or F1 to F6 from N-terminus to C-terminus, and wherein F1 to F4, F1 to F5 or F1 to F6 comprise amino acid sequences selected from the group consisting of; - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
-
Specification