Compositions and methods for promoting beta cell maturity
First Claim
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1. A method for providing an enriched population of mature, glucose-responsive insulin secreting cells, the method comprising:
- providing an initial cell population comprising at least 10% pancreatic islet-isolated insulin-expressing immature beta cells, wherein the cells do not substantially secrete insulin in response to glucose; and
contacting the initial population of immature beta cells with triiodothyronine (T3) thyroid hormone or an analog thereof under conditions and for a time sufficient to induce maturation of at least some of the cells into mature cells that secrete insulin in the presence of glucose in vitro,thereby providing an enriched population of mature, glucose-responsive insulin secreting cells.
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Abstract
Compositions and methods for providing an enriched population of mature, glucose-responsive insulin secreting cells, and for modulating insulin expression, activity and secretion in a subject.
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10 Claims
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1. A method for providing an enriched population of mature, glucose-responsive insulin secreting cells, the method comprising:
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providing an initial cell population comprising at least 10% pancreatic islet-isolated insulin-expressing immature beta cells, wherein the cells do not substantially secrete insulin in response to glucose; and contacting the initial population of immature beta cells with triiodothyronine (T3) thyroid hormone or an analog thereof under conditions and for a time sufficient to induce maturation of at least some of the cells into mature cells that secrete insulin in the presence of glucose in vitro, thereby providing an enriched population of mature, glucose-responsive insulin secreting cells.
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2. A method for providing an enriched population of mature, glucose-responsive insulin secreting cells, the method comprising:
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providing an initial cell population comprising at least 10% pancreatic islet-isolated insulin-expressing immature beta cells, wherein the cells secrete a level of insulin in the presence of glucose in vitro; and contacting the initial population of cells with triiodothyronine (T3) thyroid hormone or an analog thereof under conditions and for a time sufficient to induce maturation of at least some of the cells into mature cells that secrete a second level of insulin in the presence of glucose in vitro, wherein the second insulin level is greater than the first level, thereby providing an enriched population of mature, glucose-responsive insulin secreting cells. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10)
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