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Self-contained biological assay apparatus, methods, and applications

  • US 9,328,381 B2
  • Filed: 07/14/2015
  • Issued: 05/03/2016
  • Est. Priority Date: 10/13/2004
  • Status: Active Grant
First Claim
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1. A self-contained, biological assay apparatus, comprising:

  • a housing;

    a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing;

    a reagent supply component disposed in the housing;

    a pneumatic manifold removeably disposed in the housing in a space shared by the dispensing platform, removeably coupled to a microfluidic system having a fluidic transport layer and a plurality of reservoirs which are fluidly connected to said fluidic transport layer, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced into the fluidic transport layer are disposed in the housing in the space separate from the dispensing platform;

    a pneumatic supply system removeably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and

    a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing,wherein the apparatus is characterized as a self-contained, fully automated biological assay-performing apparatus,further wherein the test sample, once introduced, is transported from a starting location in the fluidic transport layer to the analysis reservoir separately from any other samples and separately from the pneumatic manifold and the dispensing system.

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