Automated analyzer for clinical laboratory

• US 9,329,194 B2
• Filed: 07/16/2012
• Issued: 05/03/2016
• Est. Priority Date: 11/05/2007
• Status: Active Grant

• Current Assignee: Abbott Laboratories Incorporated
• Original Assignee: Abbott Laboratories Incorporated
• Inventors: Fritchie, Patrick P., Gardner, Gregory E., Mahoney, Richard W.
• Primary Examiner(s): WRIGHT, PATRICIA KATHRYN
• Application Number:
  US13/550,240
• Publication Number:
  US 20120282684A1
• Time in Patent Office: 1,387 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 2035/00564 Handling or washing solid phase elements, e.g. beads
• CPC Class Codes: G01N 2035/0406 Individual bottles or tubes
• CPC Class Codes: G01N 2035/1051 for transporting containers, e.g. retained by friction
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C
• CPC Class Codes: G01N 35/0099 comprising robots or similar manipulators robots per se B25J
• CPC Class Codes: G01N 35/028 having reaction cells in the form of microtitration plates
• CPC Class Codes: G01N 35/1002 Reagent dispensers
• CPC Class Codes: G01N 35/1065 Multiple transfer devices
• CPC Class Codes: Y02P 20/582 Recycling of unreacted starting or intermediate materials
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/113332 with conveyance of sample along a test line in a container or rack
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A laboratory automation system that is capable of carrying out clinical chemistry assays, immunoassays, amplification of nucleic acid assays, and any combination of the foregoing, said laboratory automation system employing at least one of micro-well plates and deep multi-well plates as reaction vessels. The use of micro-well plates as reaction vessels enables the laboratory automation system to assume a variety of arrangements, i.e., the laboratory automation system can comprise a variety of functional modules that can be arranged in various ways. In order to effectively carry out immunoassays by means of micro-well plates, a technique known as inverse magnetic particle processing can be used to transfer the product(s) of immunoassays from one micro-well of a micro-well plate to another.

Claims

1. A laboratory automation system to perform clinical chemistry assays and immunoassays on a plurality multi-well plates, the system comprising:

an immunoassay module configured to perform immunoassays on a first multi-plate, the immunoassay module including;

an incubator;

a luminescence reader; and

a magnetic particle processor to mix and wash contents of the first multi-well plate;

a clinical chemistry assay module configured to perform clinical chemistry assays on a second multi-well plate, the clinical chemistry assay module including;

an absorbance reader;

a mixer; and

an incubator;

an extraction module configured to extract a nucleic acid from a biological sample and to amplify the extracted nucleic acid;

a reagent storage module including a first reagent and a second reagent;

a common platform supporting the immunoassay module, the clinical chemistry assay module, and the extraction module;

a multi-well plate storage module disposed on the platform, the multi-well plate storage module including a plurality of storage areas for storing the plurality of multi-well plates;

an aspirating/dispensing device; and

a controller configured to;

control the aspirating/dispensing device to kit the first multi-well plate by dispensing sample into the first multi-well plate and dispensing the first reagent into the first multi-well plate for an immunoassay analysis;

control the immunoassay module to analyze contents of one or more wells of the first multi-well plate by mixing, incubating, washing and reading the contents of the one or more of wells of the first multi-well plate;

control the aspirating/dispensing device to kit the second multi-well kit for a clinical chemistry assay analysis while the first multi-well plate is being analyzed by the immunoassay module, the aspirating/dispensing device to kit the second multi-well plate by dispensing sample into the second multi-well plate and dispensing the second reagent into the second multi-well plate; and

control the clinical chemistry assay module to analyze contents of one or more wells of the second multi-well plate by mixing, incubating and reading the contents of the one or more wells of the second multi-well plate while the first multi-well plate is being analyzed by the immunoassay module.

2. The laboratory automation system of claim 1, wherein the controller is further configured to control the immunoassay module to perform about two to about twenty-four immunoassays in the magnetic particle processor simultaneously.

3. The laboratory automation system of claim 1, wherein the controller is further configured to control the immunoassay module to perform about two to about 96 immunoassays in the magnetic particle processor simultaneously.

4. The laboratory automation system of claim 1 further comprising troughs having bulk liquids including at least one of a pre-trigger solution, a washer buffer or deionized water.

5. The laboratory automation system of claim 1, wherein the magnetic particle processor is a first magnetic particle processor, the extraction module including a second magnetic particle processor.

6. The laboratory automation system of claim 1, wherein the controller is further configured to vary one or more of a duration of incubation, a duration of mixing, a duration of reagent addition steps or a duration of washing of contents of one or more of the wells of the first multi-well plate.

7. The laboratory automation system of claim 1 further including a sample container, wherein the controller is further configured to control the aspirating/dispensing device to aspirate a liquid from the sample container and dispense the liquid into one or more of the first multi-well plate or the second multi-well plate.

8. The laboratory automation system of claim 1, wherein the reagent

storage module includes a first reagent container with the first reagent for the immunoassay module and a second reagent container with the second reagent for the clinical chemistry assay module.

9. The laboratory automation system of claim 8, wherein the aspirating/dispensing includes a transport mechanism and is to move one or more of a sample container, the first reagent container or the second reagent container via the transport mechanism.

10. The laboratory automation system of claim 9, wherein the luminescence reader is located outside of the magnetic particle processor, and wherein the controller is further configured to control the aspirating/dispensing device to transfer the first multi-well plate from the magnetic particle processor into the luminescence reader via the transport mechanism.

11. The laboratory automation system of claim 1 further comprising a plate rotator to rotate the second multi-well plate before processing in the clinical chemistry assay module.

12. The laboratory automation system of claim 1, wherein the controller is further configured to control the aspirating/dispensing device to kit the first multi-well plate and the second multi-well plate with the same sample.

13. The laboratory automation system of claim 1, wherein the immunoassay module comprises a chemiluminescent microparticle processor.

14. The laboratory automation system of claim 1 further comprising a second immunoassay module and a third multi-well plate, wherein the controller is configured to control the aspirating/dispensing device to kit the third multi-well plate for analysis by the second immunoassay module.

15. The laboratory automation system of claim 1, wherein the immunoassay module is located at a first location in the laboratory automation system and the clinical chemistry assay module is located at a second location in the laboratory automation system, the second location different from the first location.

16. The laboratory automation system of claim 1, wherein the kitting of the first multi-well plate is to be carried out prior to entry of the first multi-well plate into the immunoassay module and the kitting of the second multi-well plate is to be carried out prior to entry of the second multi-well plate into the clinical chemistry assay module.

17. The laboratory automation system of claim 1, wherein the luminescence reader is separate from the magnetic particle processor, the luminescence reader to read reaction mixtures in one or more of the wells of the first multi-well plate, wherein the first multi-well plate is to be transferred into the luminescence reader after processing in the magnetic particle processor.

18. The laboratory automation system of claim 17, wherein the controller is further configured to control the luminescence reader to read the reaction mixtures in one or more of the wells of the first multi-well plate while the absorbance reader is to read reaction mixtures in one or more of the wells of the second multi-well plate.

19. The laboratory automation system of claim 1 further comprising an automated robotic mechanism to transfer at least one of a reagent container or sample container from a refrigerated storage area to the immunoassay module, the clinical chemistry assay module or the extraction module of the laboratory automation system.

20. The laboratory automation system of claim 1 further comprising one or more immunoassay modules, clinical chemistry assay modules or extraction modules that are capable of being one or more of added to, subtracted from or rearranged.

21. The laboratory automation system of claim 1 further comprising a system for controlling evaporation of reagents by means of a plurality of sliding covers.

22. The laboratory automation system of claim 1, wherein, to control the clinical chemistry assay module to analyze the contents of one or more wells of the second multi-well plate, the controller is further configured to control the aspirating/dispensing device to dispense the second reagent or a third reagent into one or more wells of the second multi-well plate between readings by the absorbance reader.