Fluorochromes for organelle tracing and multi-color imaging

• US 9,334,281 B2
• Filed: 09/08/2008
• Issued: 05/10/2016
• Est. Priority Date: 09/08/2008
• Status: Active Grant

• Current Assignee: Enzo Life Sciences Incorporated (Enzo Biochem Incorporated)
• Original Assignee: Enzo Life Sciences Incorporated (Enzo Biochem Incorporated)
• Inventors: Patton, Wayne Forrest, Pande, Praveen, Xiang, Yuejun, Li, Zaiguo, Donegan, James J., Rabbani, Elazar
• Primary Examiner(s): BROOKS, CLINTON A
• Application Number:
  US12/231,988
• Publication Number:
  US 20100062429A1
• Time in Patent Office: 2,801 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: C07C 225/34 Amino anthraquinones
• CPC Class Codes: C07C 225/36 the carbon skeleton being further substituted by singly-bound oxygen atoms
• CPC Class Codes: C07C 229/18 the nitrogen atom of the amino group being further bound to carbon atoms of six-membered aromatic rings
• CPC Class Codes: C07C 2603/24 Anthracenes; Hydrogenated anthracenes
• CPC Class Codes: C07C 335/20 being further substituted by nitrogen atoms, not being part of nitro or nitroso groups
• CPC Class Codes: C07D 213/74 Amino or imino radicals substituted by hydrocarbon or substituted hydrocarbon radicals
• CPC Class Codes: C07D 295/135 with the ring nitrogen atoms and the substituent nitrogen atoms separated by carbocyclic rings or by carbon chains interrupted by carbocyclic rings
• CPC Class Codes: C07D 487/06 Peri-condensed systems
• CPC Class Codes: C07F 9/5304 Acyclic saturated phosphine oxides or thioxides
• CPC Class Codes: C07F 9/65038 condensed with carbocyclic rings or carbocyclic ring systems
• CPC Class Codes: C09B 1/005 Di-anthraquinonyl and derivative compounds
• CPC Class Codes: C09B 1/51 N-substituted amino-hydroxy anthraquinone
• CPC Class Codes: C09B 1/512 only amino and hydroxy groups
• CPC Class Codes: C09B 1/5155 only amino and hydroxy groups
• CPC Class Codes: C40B 40/04 Libraries containing only organic compounds
• CPC Class Codes: G01N 33/5005 involving human or animal cells immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025
• CPC Class Codes: G01N 33/5011 for testing antineoplastic activity

Abstract

Provided are compounds, methods and kits for identifying in cells of interest organelles including nuclei and a wide variety of organelles other than nuclei (non-nuclear organelles), as well as cell regions or cell domains. These compounds and methods can be used with other conventional detection reagents for identifying the location or position or quantity of organelles and even for distinguishing between organelles in cells of interest.

Claims

1. A compound selected from:

3. A multimeric compound comprising two or more of the compounds of claim 1 or 2 joined together through a linkage group comprising a substituted or unsubstituted alkyl group, a substituted or unsubstituted alkenyl group, a substituted or unsubstituted heteroalkyl group, a substituted or unsubstituted heteroalkenyl group comprising an amide, or one or more substituted or unsubstituted aromatic rings.

2. The compound having the structure

4. A compound comprising two organelle-targeting fluorochrome compounds having the structure:

5. The compound of claim 4 selected from

6. A compound selected from the group consisting of