Systems and methods for isolating nucleic acids from cellular samples
First Claim
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1. A method of lysing cells, comprising:
- a) contacting an aqueous biological fluid sample comprising a cell with a solution comprising beads and an aromatic solvent wherein said solvent is selected from the group consisting of toluene, ortho-xylene, meta-xylene and para-xylene; and
b) lysing said cells using a bead-beating device.
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Abstract
The present disclosure relates to systems and methods for nucleic acid isolation from cellular samples. In particular, the present disclosure provides systems and methods for lysing cells and recovering nucleic acids.
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11 Claims
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1. A method of lysing cells, comprising:
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a) contacting an aqueous biological fluid sample comprising a cell with a solution comprising beads and an aromatic solvent wherein said solvent is selected from the group consisting of toluene, ortho-xylene, meta-xylene and para-xylene; and b) lysing said cells using a bead-beating device. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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Specification