Chemistry used in biosensors
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Abstract
The invention relates to novel compositions of 1,3-disubstituted ferrocenes useful for the modification of electrodes.
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Citations
30 Claims
- 1. A ferrocene compound of formula:
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16. A device comprising an electrode comprising:
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(i) an optional self-assembled monolayer (SAM); and (ii) an electroactive active moiety (EAM), said EAM comprising a 1,3-disubstituted ferrocene. - View Dependent Claims (18, 19, 20, 21, 22, 23, 24)
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25. A device comprising an electrode comprising a self-assembled monolayer (SAM), wherein said SAM comprises a compound having the formula:
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Anchor-Spacer 1-EAM-(Spacer2)n-FG
(I),wherein said anchor comprises a cyclic-disulfide group, EAM is a 1,3-disubstituted ferrocene, FG is a functional group, Spacer 1 is a SAM forming species, Spacer 2 is a linker, and n=0 or 1.
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26. A device comprising an electrode comprising a self-assembled monolayer (SAM), wherein said SAM comprises a compound having the formula:
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Anchor-Spacer 1-EAM-Spacer 2-FG
(II),wherein said anchor is linked to said electrode group through a disulfide group, EAM is a 1,3-disubstituted ferrocene, FG is a functional group, Spacer 1 is either insulating or conducting, and Spacer 2 is an optional linker.
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27. A method for detecting one or more target analytes m a test sample, said method comprising:
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(a) contacting the test sample with a capture binding ligand under conditions such that the capture binding ligand specifically binds to a target analyst, if present, in said test sample to form a first complex, the capture binding ligand bound to a first solid support; (b) contacting said first complex, if present, to a soluble capture ligand to form a second complex, wherein said soluble capture ligand comprises a peroxide-generating system; (c) contacting said second complex with a substrate for said peroxide-generating system under conditions wherein a peroxide is generated to form an assay mixture; (d) contacting the assay mixture with a second solid support comprising an electrode comprising (i) a self-assembled monolayer (SAM) of an electroactive active moiety (EAM), or (ii) an EAM and optional SAM, wherein said EAM comprises a 1,3-disubstituted ferrocene, a self-immolative moiety, and a peroxide sensitive moiety (PSM) and has a first E0, and wherein said peroxide reacts with said PSM to release said SIM from said EAM and result in said EAM having a second E0; and
having a second E0;(f) measuring the electrochemical properties of said EAM at the first E0 and at the second E0; and (g) detecting said target analyte from said electrochemical properties. - View Dependent Claims (29, 30)
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28. A method for detecting one or more target analytes in a test sample, said method comprising:
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(a) contacting the test sample with a soluble capture ligand to form a first complex, wherein said soluble capture ligand comprises a peroxide-generating system; (b) contacting said first complex, if present, with capture binding ligand under conditions such that the capture binding ligand specifically binds to a target analyst to form a second complex, the capture binding ligand bound to a first solid support; (c) contacting said second complex with a substrate for said peroxide-generating system under conditions wherein a peroxide is generated to form an assay mixture; (d) contacting the assay mixture with a second solid support comprising an electrode comprising (i) a self-assembled monolayer (SAM) of an electroactive active moiety (EAM) or (ii) an EAM and optional SAM, wherein said EAM comprises a 1,3-disubstituted ferrocene, a self-immolative moiety, and a peroxide sensitive moiety (PSM) and has a first E0, and wherein said peroxide reacts with said PSM to release said SIM from said EAM and result in said EAM having a second E0; and
having a second E0;(f) measuring the electrochemical properties of said EAM at the first E0 and at the second E0; and (g) detecting said target analyte from said electrochemical properties.
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Specification