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Microfluidics based assay device

  • US 9,341,620 B2
  • Filed: 02/07/2012
  • Issued: 05/17/2016
  • Est. Priority Date: 02/07/2011
  • Status: Active Grant
First Claim
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1. A method of conducting an assay on a sample, the method comprising the steps of:

  • introducing a fluid sample into a microfluidic cartridge such that any analyte present in the sample is capable of being bound by an analyte binding agent and a label present in the cartridge, the cartridge comprising;

    a sample port for introducing said sample into the cartridge,a substrate comprising one or more microfluidic channels disposed therein, a binding agent within said channel(s) for binding any of said analyte within the sample, and a label for use in detecting an amount of the analyte present in the sample; and

    a detection area within or wholly comprising an area in which analyte, analyte binding agent, and label binding occurs to produce analyte binding agent/analyte/label complexes ,detecting a total level of the label that is complexed or uncomplexed to the analyte present in the detection area in order to obtain a first reference/control value;

    removing any analyte binding agent/analyte/label complexes from the detection area;

    detecting a level of any uncomplexed label and any unreacted label that remains in the detection area after removal of said analyte binding agent/analyte/label complexes; and

    determining said analyte concentration by subtracting the level of uncomplexed/unreacted label from the first reference/control value.

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