Immunoassays employing non-particulate chemiluminescent reagent
First Claim
1. A method for determining the presence and/or amount of an analyte in a sample suspected of containing the analyte, the method comprising:
- (a) providing in combination in an aqueous medium;
(i) the sample,(ii) a chemiluminescent reagent, the reagent being non-particulate and soluble in the aqueous medium and comprising a binding partner for the analyte linked to a chemiluminescent composition comprising a metal chelate and an olefinic compound, and(iii) a sensitizer reagent capable of generating singlet oxygen,(b) subjecting the combination to conditions for binding of the analyte to the binding partner for the analyte, and(c) activating the sensitizer and detecting the amount of luminescence generated by the chemiluminescent composition, the amount of luminescence being related to the amount of the analyte in the sample.
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Abstract
Methods and reagents are disclosed for conducting assays. Embodiments of the present methods and reagents are concerned with chemiluminescent reagents for determining the presence and/or amount of an analyte in a sample suspected of containing the analyte. The reagent is non-particulate and comprises a binding partner for the analyte and a chemiluminescent composition comprising an olefinic compound and a metal chelate. In embodiments of an assay, a combination is provided that comprises a sample suspected of containing the analyte, a chemiluminescent reagent as described above and a sensitizer reagent capable of generating singlet oxygen. The combination is subjected to conditions for binding of the analyte to the binding partner for the analyte. The sensitizer is activated and the amount of luminescence generated by the chemiluminescent composition is detected wherein the amount of luminescence is related to the amount of the analyte in the sample.
44 Citations
4 Claims
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1. A method for determining the presence and/or amount of an analyte in a sample suspected of containing the analyte, the method comprising:
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(a) providing in combination in an aqueous medium; (i) the sample, (ii) a chemiluminescent reagent, the reagent being non-particulate and soluble in the aqueous medium and comprising a binding partner for the analyte linked to a chemiluminescent composition comprising a metal chelate and an olefinic compound, and (iii) a sensitizer reagent capable of generating singlet oxygen, (b) subjecting the combination to conditions for binding of the analyte to the binding partner for the analyte, and (c) activating the sensitizer and detecting the amount of luminescence generated by the chemiluminescent composition, the amount of luminescence being related to the amount of the analyte in the sample. - View Dependent Claims (2, 3, 4)
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Specification