Strain of;and method for cultivating the same

• US 9,351,445 B2
• Filed: 05/16/2014
• Issued: 05/31/2016
• Est. Priority Date: 05/20/2013
• Status: Active Grant

• Current Assignee: You Song Kim
• Original Assignee: You Song Kim
• Inventors: Kim, You Song
• Primary Examiner(s): Nguyen, Son T
• Application Number:
  US14/279,970
• Publication Number:
  US 20140338257A1
• Time in Patent Office: 746 Days
• Field of Search: 47/11
• US Class Current: 1/1
• CPC Class Codes: A01G 18/20 Culture media, e.g. compost
• CPC Class Codes: A01G 18/50 Inoculation of spawn
• CPC Class Codes: A01G 18/68 Cultivation bottles

Abstract

A novel strain of Pleurotus nebrodensis (Daewang No. 1, Accession No.: KACC93181P) and a method for cultivating it are provided. The novel strain of Pleurotus nebrodensis is different from the existing Pleurotus ferulae in shape and physiological characteristic, has an extra after-ripening period, can be grown at a low temperature of 22 to 25° C. and a low water content (RH) of 60 to 65%, can be cultivated in slightly acid environment of pH 5.5 to 6.5, can utilize bottle cultivation, and has a good shape not to be easily damaged in packaging. Thus, the novel strain of Pleurotus nebrodensis according to the present invention have good commercial value, are more resistant to environmental change, and can be mass produced by automation system.

Claims

1. A method for cultivating a novel strain of Pleurotus nebrodensis, the method comprising the steps of:

1) manufacturing a medium comprising cotton seed husk of 30 to 50 vol %, corn powder of 2 to 8 vol %, wheat bran of 5 to 15 vol %, rice bran of 2 to 8 vol %, cotton seed meal of 2 to 8 vol %, corn cob of 10 to 26 vol %, sugar cane of 5 to 15 vol %, tangerine peel powder of 0.2 to 0.8 vol %, sugar of 0.2 to 0.8 vol %, soybean cake of 2 to 8 vol % and gypsum of 0.5 to 1.5 vol % (v/v %), each based on the total volume of the medium;

2) putting the medium in bottles and sterilizing by high pressure;

3) inoculating the sterilized medium with seed of Pleurotus nebrodensis and incubating the seed;

4) thinning out a part of Pleurotus nebrodensis produced in the incubated medium, and incubating the rest during an after-ripening period; and

5) growing the incubated Pleurotus nebrodensis.