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Functionalization of and use of functionalized second harmonic generating nanoprobes

  • US 9,352,055 B2
  • Filed: 09/10/2015
  • Issued: 05/31/2016
  • Est. Priority Date: 11/21/2011
  • Status: Active Grant
First Claim
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1. A method of probing structures and biological processes comprising:

  • interspersing a functionalized probe nanostructure comprising a nanocrystalline body configured with a biologically relevant functionalization molecule bound to the probe nanostructure through a functionalization platform including a plurality of modifying molecules having amine terminal groups, the modifying molecules being linked to the outer surface of the body through a plurality of hydroxyl molecules, and wherein the nanocrystalline body is formed of a crystal material having an internal crystal structure having a unit cell having no inversion symmetry such that the crystal material generates a second harmonic signal independent of the overall shape of the nanocrystalline body when illuminated by an external excitation source;

    the functionalization molecule being selected to target a species of interest, such that the functionalized probe nanostructure interacts with the targeted species of interest;

    illuminating the probe nanostructure with an external excitation source; and

    detecting the second harmonic signal from the nanoprobe.

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