Miniature HPLC device

• US 9,354,209 B2
• Filed: 06/27/2011
• Issued: 05/31/2016
• Est. Priority Date: 06/25/2010
• Status: Expired due to Fees

• Current Assignee: Imperial Innovations Limited (Imperial College London)
• Original Assignee: Imperial Innovations Limited (Imperial College London)
• Inventors: Casey, Duncan Robert, Kaplinsky, Joseph John, Salehi-Reyhani, Ali
• Primary Examiner(s): West, Paul
• Application Number:
  US13/806,233
• Publication Number:
  US 20130219999A1
• Time in Patent Office: 1,800 Days
• US Class Current: 1/1
• CPC Class Codes: F04B 19/006 Micropumps F04B43/043 and F04B43/095 take precedence
• CPC Class Codes: G01N 2030/027 Liquid chromatography
• CPC Class Codes: G01N 2030/324 speed, flow rate
• CPC Class Codes: G01N 2030/326 pumps
• CPC Class Codes: G01N 30/22 in high pressure liquid systems
• CPC Class Codes: G01N 30/32 of pressure or speed G01N30/36 takes precedence
• CPC Class Codes: G01N 30/36 in high pressure liquid systems
• CPC Class Codes: G01N 30/6095 Micromachined or nanomachined, e.g. micro- or nanosize

Abstract

A liquid chromatography device comprises one or more liquid reservoirs (3) for a liquid medium, a sample reservoir for a sample to be analyzed and a chromatography column (4) in fluid communication with the liquid reservoir (3) and the sample reservoir (5). The device further comprises a gas reservoir (1) for containing a volume of gas under pressure to force liquid from the liquid reservoir (3) through the chromatography column (4), in use.

Claims

1. A liquid chromatography device comprising:

one or more liquid reservoirs for a liquid medium,

a sample reservoir for a sample to be analysed,

a chromatography column in fluid communication with the liquid reservoir and the sample reservoir,

a pre-pressurized gas reservoir for containing a volume of gas under an initial pressure, wherein a fraction of the volume of gas released from the gas reservoir at the initial pressure is sufficient to force a first portion of liquid from the liquid reservoir through the chromatography column, in use, and

a housing containing the one or more liquid reservoirs, the sample reservoir, the chromatography column, and the gas reservoir,

wherein the volume of the gas reservoir is sized such that the volume of gas remaining within the gas reservoir, after release of the fractional volume of gas, is under a residual pressure sufficient to force a second portion of liquid from the liquid reservoir through the chromatography column.

2. A liquid chromatography device as claimed in claim 1, wherein the chromatography column is provided in a channel having a width in the range of 1 to 5,000 micrometers.

3. A liquid chromatography device as claimed in claim 2, wherein the width of the chromatography column is within the range of 20 to 5,000 micrometers.

4. A liquid chromatography device as claimed in claim 1, wherein a length of the chromatography column is in the range of 1 to 100 centimeters.

5. A liquid chromatography device as claimed in claim 4, wherein the length of the chromatography column is within the range of 2 to 100 centimeters.

6. A liquid chromatography device as claimed in claim 1 further comprising one or more optical detectors downstream of the chromatography column and arranged about a fluid channel in fluid communication with the chromatography column.

7. A liquid chromatography device as claimed in claim 6, wherein the optical detector comprises at least one LED as a light source.

8. A liquid chromatography device as claimed in claim 6, wherein the optical detector comprises opposed reflective surfaces on opposite sides of the fluid channel, the opposed reflective surfaces defining an optical cavity.

9. A liquid chromatography device as claimed in claim 8, wherein the reflective surfaces are provided as a layer on the walls of the fluid channel.

10. A liquid chromatography device as claimed in claim 6, wherein the optical detector comprises multiple light sources.

11. A liquid chromatography device as claimed in claim 1 further comprising a fluid disposal reservoir in fluid communication with the chromatography column for retaining fluid that has passed through the column for subsequent disposal.

12. A liquid chromatography device as claimed in claim 1, wherein the device is battery powered.

13. A liquid chromatography device as claimed in claim 1, wherein the device is disposable.

14. A liquid chromatography device as claimed in claim 1, wherein the device is connectable to a handheld data processing device for processing the results of the chromatography.

15. A liquid chromatography device as claimed in claim 1, wherein the device does not include a pump external to the housing to force liquid from the liquid reservoir through the chromatography column.

16. A liquid chromatography device as claimed in claim 1, wherein the volume of the gas reservoir is of sufficient size such that a fractional drop in pressure within the gas reservoir resulting from release of the fractional volume of gas to move the first portion of liquid through the chromatography column is equal to a fractional increase in volume in the gas reservoir.

17. A liquid chromatography device as claimed in claim 1, wherein the fractional drop in pressure is less than or equal to 10%.

18. A liquid chromatography device as claimed in claim 1, wherein the volume of the gas reservoir is of sufficient size such that a pressure exerted by the volume of gas contained within the gas reservoir is substantially constant when moving the liquid medium through the chromatography column.

19. A liquid chromatography device as claimed in claim 1, wherein the volume of the gas reservoir is selected within the range between 1 to 100 cubic centimeters.

20. A liquid chromatography device as claimed in claim 1, further comprising a thermometer for measuring a temperature of the device.

21. A liquid chromatography device as claimed in claim 1, further comprising a mechanism for heating or cooling the device.

22. A liquid chromatography device as claimed in claim 1, further comprising a valve to control release of gas from the gas reservoir.

23. A liquid chromatography device as claimed in claim 1 or 22, wherein the gas reservoir and the liquid reservoir are separated by a deformable membrane.

24. A liquid chromatography device as claimed in claim 1 or 22, wherein the gas reservoir and the liquid reservoir are separated by a rupturable membrane.