Compositions and methods for deploying a transgenic refuge seed blend

• US 9,365,863 B2
• Filed: 04/25/2014
• Issued: 06/14/2016
• Est. Priority Date: 05/08/2013
• Status: Active Grant

• Current Assignee: Monsanto Technology LLC (Bayer AG)
• Original Assignee: Monsanto Technology LLC (Bayer AG)
• Inventors: Burns, Wen C., Chay, Catherine A., Cloninger, Cheryl L., Deng, Mingqi, Flasinski, Stanislaw, Wu, Kunsheng
• Primary Examiner(s): Kubelik, Anne
• Assistant Examiner(s): Redden, Karen
• Application Number:
  US14/262,233
• Publication Number:
  US 20140338072A1
• Time in Patent Office: 781 Days
• US Class Current: 1/1
• CPC Class Codes: A01G 22/20 Cereals
• CPC Class Codes: C12N 15/8275 Glyphosate
• CPC Class Codes: C12N 15/8286 for insect resistance

Abstract

Methods and compositions for deploying refuge seeds with transgenic crop seeds comprising event MON 87411 are provided. The refuge seeds can be non-transgenic seeds of a similar variety to that of the transgenic crop seeds, or the refuge seeds can be a transgenic variety, but lack event MON 87411 found in the transgenic crop seeds and serve as a refuge thereto.

Claims

1. A seed blend comprising refuge corn crop seed and at least one variety of transgenic corn crop seed in a uniform mixture, wherein the mixture consists of from about 80% to about 99% first transgenic corn crop seed, wherein the first transgenic corn crop seed comprises event MON 87411 and the refuge crop seed does not comprise said event MON 87411, and wherein a representative sample of seed corn comprising the event MON 87411 has been deposited under ATCC Accession No. PTA-12669.

2. The seed blend of claim 1, wherein said mixture consists of from about 2% to about 5% refuge corn crop seed, from about 5% to about 10% refuge corn crop seed, from about 1% to about 10% refuge corn crop seed, or from about 10% to about 20% refuge corn crop seed.

3. The seed blend of claim 1, wherein the refuge corn crop seed is transgenic.

4. The seed blend of claim 3, wherein the refuge corn crop seed and the at least one variety of transgenic corn crop seed comprise a transgene that confers tolerance to at least a first herbicide.

5. The seed blend of claim 4, wherein the herbicide is selected from the group consisting of dicamba, 2,4-D, glyphosate and glufosinate.

6. The seed blend of claim 1, wherein the first transgenic corn crop seed comprises at least one additional transgenic event that confers pest resistance, and wherein the refuge corn crop seed does not comprise the at least one additional transgenic event.

7. The seed blend of claim 6, wherein the at least one additional transgenic event confers resistance to a lepidopteran pest.

8. The seed blend of claim 7, wherein the at least one additional transgenic event is selected from the group consisting of MON810, MON 89034, TC1507, MIR162, and BT11.

9. The seed blend of claim 6, wherein the at least one additional transgenic event confers resistance to a coleopteran pest.

10. The seed blend of claim 9, wherein the at least one additional transgenic event is selected from the group consisting of DAS-59122-7, MIR604, and 5307.

11. A population of plants produced by growing the seed blend of claim 1.

13. A method for deploying a refuge crop in a field of transgenic pest resistant crops comprising:

a) obtaining a seed blend according to claim 1; and

b) planting said seed blend in a field.

14. The method of claim 13, wherein said seed blend consists of from about 2% to about 5% refuge corn crop seed, from about 5% to about 10% refuge corn crop seed, from about 1% to about 10% refuge corn crop seed, or from about 10% to about 20% refuge corn crop seed.

15. The method of claim 13, wherein the refuge corn crop seed is transgenic.

16. The method of claim 15, wherein the refuge corn crop seed and the at least one variety of transgenic corn crop seed comprise a transgene that confers tolerance to at least a first herbicide.

17. The method of claim 16, wherein the herbicide is selected from the group consisting of dicamba, 2,4-D, glyphosate and glufosinate.

18. The method of claim 13, wherein the first transgenic corn crop seed comprises at least one additional transgenic event that confers pest resistance, and wherein the refuge corn crop seed does not comprise the at least one additional transgenic event.

19. The method of claim 18, wherein the at least one additional transgenic event confers resistance to a lepidopteran pest.

20. The method of claim 19, wherein the at least one additional transgenic event is selected from the group consisting of MON810, MON 89034, TC1507, MIR162, and BT11.

21. The method of claim 18, wherein the at least one additional transgenic event confers resistance to a coleopteran pest.

22. The method of claim 21, wherein the at least one additional transgenic event is selected from the group consisting of DAS-59122-7, MIR604, and 5307.

12. A method for producing a refuge crop seed blend comprising:

a) blending a first transgenic corn crop seed comprising event MON 87411 with a refuge crop corn seed lacking said event MON 87411; and

b) ensuring a uniform mixture of the first transgenic corn crop seed and the refuge corn crop seed is provided to produce a refuge crop seed blend,

wherein the mixture consists of from about 80% to about 99% first transgenic corn crop seed, and wherein a representative sample of seed corn comprising event MON 87411 has been deposited under ATCC Accession No. PTA-12669.