Immobilization support, process for producing the same, electrode, process for producing the same, electrode reaction utilizing apparatus and process for producing the same

• US 9,365,882 B2
• Filed: 02/27/2009
• Issued: 06/14/2016
• Est. Priority Date: 06/05/2003
• Status: Active Grant

• Current Assignee: Murata Manufacturing Co Limited
• Original Assignee: Sony Corporation (Sony Group Corp.)
• Inventors: Sato, Atsushi, Ikeda, Tokuji, Kano, Kenji
• Primary Examiner(s): Carlson, Karen Cochrane
• Assistant Examiner(s): Tichy, Jennifer M. H.
• Application Number:
  US12/394,975
• Publication Number:
  US 20090159438A1
• Time in Patent Office: 2,664 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: C12Q 1/003 Functionalisation
• CPC Class Codes: H01M 4/86 Inert electrodes with catalytic activity, e.g. for fuel cells
• CPC Class Codes: H01M 8/16 Biochemical fuel cells, i.e. cells in which microorganisms function as catalysts
• CPC Class Codes: Y02E 60/50 Fuel cells
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

An immobilization carrier containing an electron acceptor compound is used in addition to glutaraldehyde and poly-L-lysine to immobilize an enzyme and an electron acceptor compound simultaneously to an electrode. For example, here are used diaphorase as the enzyme and 2-amino-3-carboxy-1,4-naphthoquinone (ACNQ) as the electron acceptor compound.

Claims

1. An electrode comprising:

an immobilization carrier including

glutaraldehyde, poly-L-lysine,

an electron acceptor compound wherein the electron acceptor compound has at least one amino group, and

an enzyme, and further wherein the electron acceptor compound is combined with a cross-linking agent and the electron acceptor compound functions to support cross-linking between glutaraldehyde and poly-L-lysine, and further wherein the enzyme and electron acceptor compound are immobilized simultaneously to form an enzyme-immobilized electrode, and an aldehyde group at one end of the glutaraldehyde and an amino group of the poly-L-lysine form and amide linkage and an aldehyde group at another end of the glutaraldehyde and the amino group of the enzyme simultaneously form an amide linkage to cross-link.

2. The electrode according to claim 1, further comprising a coenzyme.

3. The electrode according to claim 2, wherein the coenzyme has at least one amino group.