Histological specimen treatment apparatus and method

• US 9,366,605 B2
• Filed: 02/14/2012
• Issued: 06/14/2016
• Est. Priority Date: 05/28/2008
• Status: Active Grant

• Current Assignee: Steven Paul Wheeler
• Original Assignee: Steven Paul Wheeler
• Inventors: Wheeler, Steven Paul
• Primary Examiner(s): Crow, Robert T
• Assistant Examiner(s): Dauner, Joseph G
• Application Number:
  US13/396,541
• Publication Number:
  US 20120202241A1
• Time in Patent Office: 1,582 Days
• Field of Search: 219/678
• US Class Current: 1/1
• CPC Class Codes: G01N 1/31 Apparatus therefor
• CPC Class Codes: G01N 1/36 Embedding or analogous mounting of samples

Abstract

Method and apparatus for processing tissue specimens against decomposition, putrefaction and autolysis which use a simple three step procedure in a single vessel or container. First, the specimens are saturated with a solvent mixture of a ketone and a hydrocarbon, e.g., an Acetone/Hexane or an Acetone/Xylene mixture, to dissolve lipids and other cellular solutes. The container is then flooded with melted Paraffin. In a last step, the solvent mixture is vaporized and evacuated from the container, allowing the melted Paraffin to replace the vaporized solvent and impregnate the specimens. Raw, i.e., non-processed and non-burred specimens up to 5 mm thick can be processed in about 60 minutes. A solvent regenerator distills the evacuated solvent; and converts vent waste gases into carbon dioxide and water through a thermocatalytic oxidizer.

Claims

1. A specimen processing method comprising:

treating a specimen in a pressure and temperature controllable reaction container to remove solutes from the specimen and replace the solutes with preserving compound, comprising;

temperature and pressure conditioning a dissolving compound in a solvent vessel;

transferring the temperature and pressure conditioned dissolving compound from the solvent vessel to the reaction container, the dissolving compound flowing into the reaction container being in a heated and pressurized condition, the dissolving compound penetrating the specimen and diluting solutes therein, wherein pressure regulation is provided between the solvent vessel and the reaction container during the transferring;

introducing a preserving compound into the reaction container;

vaporizing the dissolving compound;

evacuating the dissolving compound and diluted solutes from the reaction container; and

reducing the temperature in the reaction container below the melting point of the preserving compound.

2. The method of claim 1 wherein the dissolving compound in the reaction container comprises at least one of azeotropic and zeotropic dehydrant mixtures.

3. The method of claim 1 wherein the dissolving compound comprises a solvent including at least one of ketones, esters, alcohols, aldehydes, ethers, aromatics, low molecular weight hydrocarbons, and a mixture of low molecular weight hydrocarbons.

4. The method of claim 1 wherein the dissolving compound in the reaction container comprises an azeotrope.

5. The method of claim 1 performed without microwave heating.

6. The method of claim 1 further comprising regenerating an amount of the dissolving compound evacuated from the container in a regeneration canister.

7. The method of claim 6 wherein the regenerating comprises distilling the evacuated compound, and converting vent waste gases into carbon dioxide and water through a thermocatalytic oxidizer.

8. The method of claim 6 further comprising conditioning regenerated dissolving compound for introduction into the reaction container.

9. The method of claim 8 wherein the conditioning comprises heating the regenerated dissolving compound to a temperature of approximately 60°

C., and establishing a pressure of approximately 0.8 bars.

10. The method of claim 1 wherein the dissolving compound penetrates the specimen and dilutes solutes therein for no more than approximately 30 minutes, and the preserving compound impregnates the specimen for no more than approximately 30 minutes.

11. The method of claim 10 wherein the specimen comprises non-processed and non-burred tissue sections up to 5 mm thick.

12. The method of claim 1 wherein the dissolving compound penetrates the specimen and dilutes solutes therein for no more than approximately 50 minutes, and the preserving compound impregnates the specimen for no more than approximately 50 minutes;

and the specimen comprises non-processed and non-burred tissue sections up to 10 mm thick.

13. The method of claim 1 further comprising admitting the liquid preserving compound into the container before introducing the dissolving compound.

14. The method of claim 1 wherein the dissolving compound has a refractive index between about 1.350 and 1.499 at 20°

C.

15. The method of claim 1 wherein the dissolving compound comprises a lubricant for process pumps and valves.

16. The method of claim 1 wherein the dissolving compound comprises a mixture of approximately 17% to 65% per weight Acetone and 16% to 40% per weight of at least one Hexane.

17. The method of claim 1 wherein the dissolving compound is vaporizable at a temperature in the range of 30 to 100°

C.

18. The method of claim 17 wherein the dissolving compound has a refractive index between about 1.350 and 1.499 at 20°

C.

19. The method of claim 1 wherein the reaction container is pressurized during diluting of the solutes.

20. The method of claim 1 wherein pressure regulation is provided by a valve located in a fluid line connecting the solvent vessel and the reaction container.

21. The method of claim 1 wherein the solutes comprise lipids.