Integrated systems for the multiplexed amplification and detection of six and greater dye labeled fragments

• US 9,366,631 B2
• Filed: 07/27/2011
• Issued: 06/14/2016
• Est. Priority Date: 04/04/2007
• Status: Active Grant

• Current Assignee: ANDE Corp.
• Original Assignee: NetBio, Inc.
• Inventors: Tan, Eugene, Lam, Heung Chuan, Bogdanov, Valery Leonidovich, Kellogg, Gregory John, Wright, John A., Thomann, Ulrich Hans, Selden, Richard F.
• Primary Examiner(s): Lapage, Michael P
• Application Number:
  US13/191,952
• Publication Number:
  US 20130155403A1
• Time in Patent Office: 1,784 Days
• Field of Search: 356/311, 356/317, 356/416, 356/417, 422 8207- 8208, 436/172, 2504581-4612
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/0684 Venting, avoiding backpressure, avoid gas bubbles
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/147 Employing temperature sensors
• CPC Class Codes: B01L 2300/0627 Sensor or part of a sensor is integrated
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/069 Absorbents; Gels to retain a fluid
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2300/16 Surface properties and coatings
• CPC Class Codes: B01L 2300/1822 using Peltier elements
• CPC Class Codes: B01L 2300/1844 using fans
• CPC Class Codes: B01L 2300/1894 Cooling means; Cryo cooling
• CPC Class Codes: B01L 2400/0421 electrophoretic flow
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502753 characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation separation in general B01D; microapparatus for analysis using electrophoresis G01N27/44791; sample preparation G01N1/28
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]
• CPC Class Codes: C12Q 1/6869 : Methods for sequencing
• CPC Class Codes: G01N 2021/6441 : with two or more labels
• CPC Class Codes: G01N 21/6402 : Atomic fluorescence; Laser induced fluorescence
• CPC Class Codes: G01N 21/6428 : Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6452 : Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/6486 : Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence
• CPC Class Codes: G01N 2201/06113 : Coherent sources; lasers
• CPC Class Codes: G01N 27/44726 : using specific dyes, markers or binding molecules
• CPC Class Codes: G01N 27/44743 : Introducing samples
• CPC Class Codes: G01N 27/44782 : of a plurality of samples
• CPC Class Codes: G01N 27/44791 : Microapparatus sample containers with integrated microfluidic structures B01L3/5027
• CPC Class Codes: Y10T 436/2575 : Volumetric liquid transfer

Abstract

The present disclosure provides fully integrated microfluidic systems to perform nucleic acid analysis. These processes include sample collection, nucleic acid extraction and purification, amplification, sequencing, and separation and detection. The present disclosure also provides optical detection systems and methods for separation and detection of biological molecules. In particular, the various aspects of the invention enable the simultaneous separation and detection of a plurality of biological molecules, typically fluorescent dye-labeled nucleic acids, within one or a plurality of microfluidic chambers or channels. The nucleic acids can be labeled with at least 6 dyes, each having a unique peak emission wavelength. The present systems and methods are particularly useful for DNA fragment sizing applications such as human identification by genetic fingerprinting and DNA sequencing applications such as clinical diagnostics.

Claims

1. A system to detect components separated by electrophoresis comprising:

(i) an integrated biochip comprising a thermal cycling chamber adapted for multiplexed amplification in one PCR reaction, said thermal cycling chamber in fluid communication with at least one channel on said biochip, wherein each channel comprises a detection position;

(ii) primers labeled with 6 or more fluorescent dyes for generating amplified nucleic acid fragments labeled with said 6 or more fluorescent dyes;

(iii) no more than one light source positioned for illuminating said plurality of detection positions over a physical space on said biochip;

(iv) one or a plurality of first optical elements selected from the group consisting of lenses, pinholes, mirrors and objectives, positioned over said physical space for collecting and directing light emanating from the plurality of detection positions on said substrate;

(v) a scanning mirror galvanometer for sequentially illuminating and collecting light from said light source at each one of said plurality of detection positions; and

(vi) a wavelength dispersive element selected from the group consisting of prisms, diffraction gratings, transmission gratings, holographic diffraction gratings and spectrographs positioned to disperse the wavelength components of the fluorescence over a physical space according to light wavelength, and to provide at least a portion of the dispersed wavelength components by reflecting a predetermined wavelength of light which essentially corresponds to a fluorescence emission maximum of a fluorescent dye present in at least one of the detection positions to at least six detectors or a multi-element PMT positioned to detect fluorescence from said labeled amplified nucleic acid fragments.

2. The system of claim 1, wherein the light source is a laser.

3. The system of claim 1, wherein each detector is capable of detecting light selected from the group consisting of:

ultraviolet light, visible light, infrared light, and combinations thereof.

4. The system of claim 1, wherein said physical space on said biochip is plastic.

5. A system to simultaneously detect fragments from sequencing of at least two DNA templates in a single separation channel, comprising:

(i) an integrated biochip comprising at least one thermal cycling chamber adapted for multiplexed amplification in one PCR reaction, said thermal cycling chamber in fluid communication with at least one channel on said biochip, wherein each channel comprises a detection position;

(ii) primers labeled with 8 or more fluorescent dyes for generating two sets of nucleic acid fragments labeled with said 8 or more fluorescent dyes, said dyes being members of at least two 4-dye containing sets, such that the emission wavelengths of the dyes in the at least two sets are different;

(iii) no more than one light source positioned for illuminating said plurality of detection positions over a physical space on said biochip;

(iv) one or a plurality of first optical elements selected from the group consisting of lenses, pinholes, mirrors and objectives, positioned over said physical space for collecting and directing light emanating from the plurality of detection positions on said biochip;

(v) a scanning mirror galvanometer for sequentially illuminating and collecting light from said light source at each one of said plurality of detection positions; and

vi) a wavelength dispersive element selected from the group consisting of prisms, diffraction gratings, transmission gratings, holographic gratings and spectrographs positioned to disperse the wavelength components of the fluorescence over a physical space according to light wavelength, and to provide at least a portion of the dispersed wavelength components to at least eight detectors or a multi-element PMT positioned to detect fluorescence from said labeled amplified nucleic acid fragments.

6. The system of claim 5, in which the number of sequenced fragments detected simultaneously is at least three, the number of 4-dye sets is at least three, and the number of fluorescent dyes is at least 12.

7. The system of claim 5, in which the number of sequenced fragments detected simultaneously is at least four, the number of 4-dye sets is at least four, and the number of fluorescent dyes is at least 16.

8. The system of claim 5, in which the number of sequenced fragments detected simultaneously is at least 5, the number of 4-dye sets is at least five and the number of fluorescent dyes is at least 20.

9. The system of claim 5, wherein said physical space on said biochip is plastic.

10. A system to detect components separated by electrophoresis comprising:

(i) a biochip comprising a thermal cycling chamber adapted for multiplexed amplification in one PCR reaction;

(ii) primers labeled with 6 or more fluorescent dyes for generating amplified nucleic acid fragments labeled with said 6 or more fluorescent dyes;

(iii) a second chamber adapted for additional processing, said processing selected from the group consisting of;

nucleic acid extraction;

nucleic acid purification;

pre-nucleic acid amplification cleanup;

post-nucleic acid amplification cleanup;

pre-nucleic acid sequencing cleanup;

nucleic acid sequencing;

post-nucleic acid sequencing cleanup;

reverse transcription;

pre-reverse transcription cleanup;

post-reverse transcription cleanup;

nucleic acid ligation;

nucleic acid hybridization; and

quantification; and

(iv) no more than one light source positioned for illuminating a plurality of detection positions over a physical space on said biochip;

(v) one or a plurality of first optical elements selected from the group consisting of lenses, pinholes, mirrors and objectives positioned over said physical space for collecting and directing light emanating from the plurality of detection positions on said substrate;

(vi) a scanning mirror galvanometer for sequentially illuminating and collecting light from said light source at each one of said plurality of detection positions; and

(vii) a wavelength dispersive element selected from the group consisting of prisms, diffraction gratings, transmission gratings, holographic diffraction gratings and spectrographs positioned to disperse the wavelength components of the fluorescence over a physical space according to light wavelength, and to provide at least a portion of the dispersed wavelength components to at least six detectors or a multi-element PMT, positioned to detect fluorescence from said labeled amplified nucleic acid fragments.

11. The system of claim 10, wherein the light source is a laser.

12. The system of claim 10, wherein each detector is capable of detecting light selected from the group consisting of:

ultraviolet light, visible light, infrared light, and combinations thereof.

13. The system of claim 10, wherein said physical space on said biochip is plastic.