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Digital analyte analysis

  • US 9,366,632 B2
  • Filed: 04/19/2013
  • Issued: 06/14/2016
  • Est. Priority Date: 02/12/2010
  • Status: Active Grant
First Claim
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1. A method for detecting target sequences from a sample, the method comprising:

  • providing a plurality of droplets each comprising a nucleic acid molecule, reagents for an amplification reaction, and a plurality of different optically labeled probes, each specific for a different target sequence;

    amplifying a target sequence from the nucleic acid molecule in the droplets;

    flowing the droplets past a detector that detects polarized light; and

    detecting a level of polarized light emitted from an optically labeled probe that identifies the target sequence in each of the droplets.

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