Oxidation-reduction potential test device including a multiple layer gel
First Claim
1. An oxidation-reduction potential test device, comprising:
- a substrate;
a spacer layer interconnected to at least a portion of the substrate, wherein the spacer layer defines a sample chamber;
a test strip overlay, wherein the test strip overlay is interconnected to at least a portion of the spacer layer;
a first test lead supported by the substrate, the first test lead including;
a first area extending into the sample chamber;
a second area extending from the sample chamber to a readout region;
a second test lead supported by the substrate, the second test lead including;
a first area extending into the sample chamber;
a second area extending from the sample chamber to a readout region;
a reference cell;
a reference lead, including;
a first area in electrical contact with the reference cell;
a second area extending from the reference cell to a readout region;
a filter, wherein at least a portion of the filter overlaps at least a portion of each of the first area of the first test lead, the first area of the second test lead, and the first area of the reference lead; and
a gel volume, wherein the gel volume includes a first layer in contact with the reference cell and a second layer over the first layer, wherein the first layer differs in composition from the second layer, wherein the first layer comprises an agarose and hydroxyethyl cellulose layer, and wherein the second layer comprises an agarose and KCl layer.
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Accused Products
Abstract
A multiple layer gel and method for forming a multiple layer gel are provided. The multiple layer gel includes an isolation layer and an electrolyte layer. The isolation layer provides a molecular weight screen, to prevent proteins or other molecules from contacting a reference cell covered by the isolation layer. The electrolyte layer covers the isolation layer, and provides a source of ions that place the reference cell in ionic and/or electrical contact with a fluid sample. The multiple layer gel can be used to maintain a reliable reference voltage from an associated reference cell while an electrical potential or other electrical characteristic of a sample fluid is being determined.
84 Citations
29 Claims
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1. An oxidation-reduction potential test device, comprising:
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a substrate; a spacer layer interconnected to at least a portion of the substrate, wherein the spacer layer defines a sample chamber; a test strip overlay, wherein the test strip overlay is interconnected to at least a portion of the spacer layer; a first test lead supported by the substrate, the first test lead including; a first area extending into the sample chamber; a second area extending from the sample chamber to a readout region; a second test lead supported by the substrate, the second test lead including; a first area extending into the sample chamber; a second area extending from the sample chamber to a readout region; a reference cell; a reference lead, including; a first area in electrical contact with the reference cell; a second area extending from the reference cell to a readout region; a filter, wherein at least a portion of the filter overlaps at least a portion of each of the first area of the first test lead, the first area of the second test lead, and the first area of the reference lead; and a gel volume, wherein the gel volume includes a first layer in contact with the reference cell and a second layer over the first layer, wherein the first layer differs in composition from the second layer, wherein the first layer comprises an agarose and hydroxyethyl cellulose layer, and wherein the second layer comprises an agarose and KCl layer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. An oxidation-reduction potential test device, comprising:
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a substrate; a spacer layer interconnected to at least a portion of the substrate, wherein the spacer layer defines a sample chamber; a test strip overlay, wherein the test strip overlay is interconnected to at least a portion of the spacer layer; a first test lead supported by the substrate, the first test lead including; a first area extending into the sample chamber; a second area extending from the sample chamber to a readout region; a second test lead supported by the substrate, the second test lead including; a first area extending into the sample chamber; a second area extending from the sample chamber to a readout region; a reference cell; a reference lead, including; a first area in electrical contact with the reference cell; a second area extending from the reference cell to a readout region; a filter, wherein at least a portion of the filter overlaps at least a portion of each of the first area of the first test lead, the first area of the second test lead, and the first area of the reference lead; and a gel volume, wherein the gel volume includes a first layer in contact with the reference cell and a second layer over the first layer, wherein the first layer differs in composition from the second layer, wherein the first layer comprises about a water based gel containing about 4% agarose and about 1% hydroxyethyl cellulose, and wherein the second layer comprises a water based gel containing about 4% agarose and about a 3M concentration of KCl. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18)
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19. An oxidation-reduction potential test device, comprising:
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a substrate; a spacer layer interconnected to at least a portion of the substrate, wherein the spacer layer defines a sample chamber; a test strip overlay, wherein the test strip overlay is interconnected to at least a portion of the spacer layer; a first test lead supported by the substrate, the first test lead including; a first area extending into the sample chamber; a second area extending from the sample chamber to a readout region; a second test lead supported by the substrate, the second test lead including; a first area extending into the sample chamber; a second area extending from the sample chamber to a readout region; a reference cell; a reference lead, including; a first area in electrical contact with the reference cell; a second area extending from the reference cell to a readout region; a filter, wherein at least a portion of the filter overlaps at least a portion of each of the first area of the first test lead, the first area of the second test lead, and the first area of the reference lead; and a gel volume, wherein the gel volume includes a first layer in contact with the reference cell and a second layer over the first layer, wherein the first layer differs in composition from the second layer, wherein the first layer is an isolation layer, and wherein the second layer is an electrolyte layer. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29)
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Specification