Production of recombinant proteins with simple glycoforms
First Claim
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1. A genetically modified cell line deficient in mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I (Mgat1) and glutamine synthetase (GS), wherein the deficiency in Mgat1 is due to a deletion of less than 200 base pairs, with an optional insertion in addition to the deletion, within the coding region of the chromosomal sequence encoding Mgat1.
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Abstract
The present invention provides cell lines deficient in mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I (Mgat1). Also provided are methods for producing the Mga1 deficient cell lines and methods for using the Mgat1 deficient cell lines for the production of recombinant proteins having simple glycoforms.
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- 1. A genetically modified cell line deficient in mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I (Mgat1) and glutamine synthetase (GS), wherein the deficiency in Mgat1 is due to a deletion of less than 200 base pairs, with an optional insertion in addition to the deletion, within the coding region of the chromosomal sequence encoding Mgat1.
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