Method for producing intestinal cells
First Claim
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1. A method of producing intestinal cells expressing Cdx2, comprising the steps of:
- (a) obtaining mouse or human embryonic stem (ES) cells,(b) culturing the ES cells of step (a) with Activin and/or bFGF to produce definitive endoderm cells,(c) culturing the definitive endoderm cells of step (b) with (2′
Z,3′
E)-6-bromoindirubin-3′
-oxime (BIO) and N-[(3,5-difluorophenyl)acetyl]-L-Ala-2-phenyl-L-Gly-tert-butyl-OH (DAPT) and in the presence of M15 cells or MEF cells, to thereby induce differentiation of the definitive endoderm cells into intestinal cells expressing Cdx2.
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Abstract
An object of the present invention is to provide a method of producing intestinal cells by use of pluripotent stem cells as a starting material. According to the present invention, provided is a method of producing intestinal cells, comprising the steps of: (A) inducing differentiation of pluripotent stem cells into definitive endoderm cells; and (B) culturing the definitive endoderm cells in the presence of (2′Z,3′E)-6-bromoindirubin-3′-oxime (BIO) and N-[(3,5-difluorophenyl)acetyl]-L-Ala-2-phenyl-L-Gly-tert-butyl-OH (DAPT) to thereby induce differentiation of the definitive endoderm cells into intestinal cells.
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Citations
4 Claims
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1. A method of producing intestinal cells expressing Cdx2, comprising the steps of:
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(a) obtaining mouse or human embryonic stem (ES) cells, (b) culturing the ES cells of step (a) with Activin and/or bFGF to produce definitive endoderm cells, (c) culturing the definitive endoderm cells of step (b) with (2′
Z,3′
E)-6-bromoindirubin-3′
-oxime (BIO) and N-[(3,5-difluorophenyl)acetyl]-L-Ala-2-phenyl-L-Gly-tert-butyl-OH (DAPT) and in the presence of M15 cells or MEF cells, to thereby induce differentiation of the definitive endoderm cells into intestinal cells expressing Cdx2. - View Dependent Claims (2, 3, 4)
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Specification