Method of preparing libraries of template polynucleotides
First Claim
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1. A library of different target nucleic acids comprising a plurality of different polynucleotide duplexes having polynucleotide adapters at each end;
- wherein polynucleotide adapters are forked adapters formed by annealing of partially complementary first and second polynucleotide strands;
wherein the first polynucleotide strand comprises the sequence set forth in SEQ ID NO;
1; and
wherein the second polynucleotide strand comprises the sequence set forth in SEQ ID NO;
2.
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Abstract
The present invention relates to a method for preparing a library of template polynucleotides and use thereof in methods of solid-phase nucleic acid amplification. More specifically, the invention relates to a method for preparing a library of template polynucleotides that have common sequences at their 5′ ends and at their 3′ ends.
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Citations
4 Claims
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1. A library of different target nucleic acids comprising a plurality of different polynucleotide duplexes having polynucleotide adapters at each end;
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wherein polynucleotide adapters are forked adapters formed by annealing of partially complementary first and second polynucleotide strands; wherein the first polynucleotide strand comprises the sequence set forth in SEQ ID NO;
1; andwherein the second polynucleotide strand comprises the sequence set forth in SEQ ID NO;
2. - View Dependent Claims (2, 3, 4)
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Specification