On-farm integrated high-solids processing system for biomass

• US 9,376,697 B2
• Filed: 10/25/2013
• Issued: 06/28/2016
• Est. Priority Date: 05/01/2013
• Status: Active Grant

• Current Assignee: United States Department Of Agriculture
• Original Assignee: University Of Kentucky Research Foundation (University of Kentucky)
• Inventors: Nokes, Sue E., Lynn, Bert C., Rankin, Stephen, Knutson, Barbara, Montross, Michael D., Flythe, Michael
• Primary Examiner(s): Harward, Soren
• Assistant Examiner(s): Pyla, Paul D.
• Application Number:
  US14/063,616
• Publication Number:
  US 20140329285A1
• Time in Patent Office: 977 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: C12N 9/2437 Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
• CPC Class Codes: C12P 19/02 Monosaccharides 2-ketogulonic acid C12P7/60
• CPC Class Codes: C12P 19/14 produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase , e.g. by cellulase, hemicellulase
• CPC Class Codes: C12P 21/00 Preparation of peptides or proteins single cell protein C12N1/00
• CPC Class Codes: C12P 2201/00 Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
• CPC Class Codes: C12P 2203/00 Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source ethanol C12P7/10
• CPC Class Codes: C12P 39/00 Processes involving microorganisms of different genera in the same process, simultaneously
• CPC Class Codes: C12P 7/10 substrate containing cellulosic material
• CPC Class Codes: C12P 7/16 Butanols
• CPC Class Codes: C12P 7/28 Acetone-containing products
• CPC Class Codes: C12P 7/52 Propionic acid; Butyric acids
• CPC Class Codes: C12P 7/54 Acetic acid vinegar C12J
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel
• CPC Class Codes: Y02P 20/52 using catalysts, e.g. selective catalysts

Abstract

A method for on-farm processing a biomass feedstock into a useful industrial chemicals includes the steps of (a) delignifying the biomass feedstock to produce a delignified biomass, (b) subjecting the delignified biomass to cellulase production, (c) subjecting the delignified biomass with attached cellulase to simultaneous cellulolytic and solventogenic reactions to produce useful industrial chemicals (d) collecting and separating the useful industrial chemical from the fermentation broth and (e) collecting the fermentation residues.

Claims

1. A method of on-farm processing a biomass feedstock into useful industrial chemicals, comprising:

(a) placing a non-sterilized biomass feedstock at a density of approximately 11 lb/ft³ to 21 lb/ft³ or maintained in bales on a false floor within a single sealed vessel with an overhead delivery system and delignifiying said biomass feedstock by adding a transition metal and a peroxide to produce a delignified biomass;

(b) subjecting said delignified biomass from (a) to a solid-substrate reaction to produce cellulase, said solid-substrate reaction comprising inoculating the delignified biomass through the overhead delivery system with a thermophilic anaerobic microorganism and maintaining a first temperature range of 50-65°

C. within the single sealed vessel to produce a delignified biomass with attached cellulase and to produce fermentation residues;

(c) subjecting said delignified biomass with attached cellulase from (b) to a simultaneous cellulolytic and solventogenic solid-substrate reaction by inoculating the delignified biomass with attached cellulase of (b) with an anaerobic microorganism through the overhead delivery system and maintaining the single sealed vessel at a second temperature range of between 25-40°

C. under anaerobic conditions to produce industrial chemicals and fermentation residues; and

(d) percolating a media from the overhead delivery system through the biomass to remove fermentation residues and industrial chemicals during (b) and (c) and collecting said percolated media from beneath the false floor and separating industrial chemicals from said media and recycling the media back to the overhead delivery system.

2. The method of claim 1, including repeating any or all of steps (a)-(d), and then (e) collecting and using said fermentation residues on the farm.

3. The method of claim 2, wherein the transition metal is iron.

4. The method of claim 1, wherein said thermophilic anaerobic microorganism is a thermophile.

5. The method of claim 1, wherein the thermophilic anaerobic microorganism is selected from the group consisting of Clostridium, Thermobifida, Fibrobacter, Ruminococcus, Butyrivibrio and combinations thereof.

6. The method of claim 1, wherein the thermophilic anaerobic microorganism is selected from the group consisting of Clostridium thermocellum, Clostridium cellulolyticum, Clostridium cellulovorans, Clostridium lentocellum, Thermobifida fusca, Thermobifida cellulosilytica, Thermobifida alba, Fibrobacter succinogenes, Fibrobacter intestinalis, Ruminococcus albus, Ruminococcus flavefaciens, Ruminococcus champanellensis, Ruminococcus bromii, Ruminococcus gauvreauii, Ruminococcus lactaris, Butyrivibrio fibrisolvens, Butyrivibrio hungatei, Butyrivibrio crossotus, Butyrivibrio proteoclastic and combinations thereof.

7. The method of claim 1, wherein the anaerobic microorganism is selected from the group consisting of Clostridium, Saccharomyces and combinations thereof.

8. The method of claim 7, wherein the anaerobic microorganism is selected from the group consisting of Clostridium thermocellum, Clostridium acetobutylicum, Clostridium beijerinkii, Clostridium bifermentans, Clostridium kluyveri, Clostridium ljungdahii, Clostridium saccharolyticum, Clostridium saccharoperbutylacetonicum, Clostridium sporogenes, Saccharomyces boulardii, Saccharomyces arboricola, Saccharomyces bayanus, Saccharomyces cariocanus, Saccharomyces cariocus, Saccharomyces carlsbergensis, Saccharomyces castelli, Saccharomyces cerevisiae and combinations thereof.

9. The method of claim 8 including percolating water or media through said biomass feedstock as it is undergoing hydrolysis/solventogenesis so as to remove organic acids and solvents and maintain favorable anaerobic organism growth conditions for solventogenic reaction.

10. The method of claim 8, including holding said biomass feedstock in said vessel, maintaining a first set of reaction conditions in said vessel when subjecting said delignified biomass to cellulase production reaction and maintaining a second, different set of reaction conditions in said vessel when said delignified biomass with attached cellulase are subjected to simultaneous cellulolytic and solventogenic reactions.

11. The method of claim 10 including establishing said first and second set of reaction conditions by controlling an environmental condition, wherein the environmental condition is selected from the group consisting of chemical pretreatment, active temperature control, oxygen content, pH and a combination thereof to repress competing microorganisms.

12. The method of claim 10, wherein first set of reaction conditions include an anaerobic environment with thermophilic temperatures of between 50°

C. and 65°

C. and pH appropriate to the cellulase producing microorganism, and said second set of reaction conditions include an anaerobic environment with mesophilic temperatures of between 25°

C. and 40°

C. and pH appropriate to the solventogenic microorganism.

13. The method of claim 12, wherein said vessel in which said reactions occur is an agricultural bulk storage system.

14. The method of claim 13, further including removing (a) lignin degradation products, (b) fermentation products derived from cellulase production and (c) fermentation products derived from solventogenesis from said vessel.

15. The method of claim 14 including removing said fermentation products by membrane separation, wherein the membrane comprises a material selected from the group consisting of a hydrophobic nonporous membrane into which solvents dissolve selectively, a poly(dimethylsiloxane) membrane, a poly(tetrafluoroethylene) membrane, a poly(vinylidene fluoride) membrane, a porous membrane functionalized to promote pervaporation or perstraction of fementation-derived solvents, a poly(vinylidene fluoride) membrane, a poly(ethersulfone) membrane, an anodized alumina membrane, a track-etched polycarbonate membrane, a mesoporous silica-based membrane derived by surfactant templating and combinations thereof.

16. The method of claim 13 including returning fermentation residues to said farm by feeding to animals on said farm, gasifying at said farm, combusting at said farm or applying to land at said farm.