Mutant DNA polymerases
First Claim
Patent Images
1. An isolated mutant Tfi DNA polymerase comprising an amino acid sequence having at least 95% identity to SEQ ID NO:
- 2 with a D→
A point mutation at amino acid position 144 of SEQ ID NO;
2 and an E→
D point mutation at position 437 of SEQ ID NO;
2.
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Accused Products
Abstract
An isolated mutant Tfi DNA polymerase having a D144A point mutation. This polymerase may be used in methods including, but not limited to, nucleic acid synthesis, DNA sequencing, nucleic acid amplification and cDNA synthesis.
29 Citations
12 Claims
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1. An isolated mutant Tfi DNA polymerase comprising an amino acid sequence having at least 95% identity to SEQ ID NO:
- 2 with a D→
A point mutation at amino acid position 144 of SEQ ID NO;
2 and an E→
D point mutation at position 437 of SEQ ID NO;
2. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
- 2 with a D→
Specification