Strategies for high throughput identification and detection of polymorphisms
First Claim
1. A method for identifying one or more polymorphisms in nucleic acid samples, comprising the steps of:
- (a) providing a plurality of nucleic acid samples of interest;
(b) performing a complexity reduction on each of the samples to provide a plurality of libraries of the nucleic acid samples,(c) ligating sequencing adapters carrying a 3′
-T overhang to the complexity reduced nucleic acid samples in the libraries and combining the libraries;
(d) sequencing at least a portion of the combined libraries to obtain sequences;
(e) aligning each of the sequences obtained in step (d) to obtain an alignment; and
(f) determining one or more polymorphisms between the plurality of nucleic acid samples in the alignment of step (e).
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Abstract
The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.
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Citations
12 Claims
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1. A method for identifying one or more polymorphisms in nucleic acid samples, comprising the steps of:
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(a) providing a plurality of nucleic acid samples of interest; (b) performing a complexity reduction on each of the samples to provide a plurality of libraries of the nucleic acid samples, (c) ligating sequencing adapters carrying a 3′
-T overhang to the complexity reduced nucleic acid samples in the libraries and combining the libraries;(d) sequencing at least a portion of the combined libraries to obtain sequences; (e) aligning each of the sequences obtained in step (d) to obtain an alignment; and (f) determining one or more polymorphisms between the plurality of nucleic acid samples in the alignment of step (e). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A method for identifying one or more polymorphisms in nucleic acid samples, comprising:
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(a) performing a complexity reduction on a plurality of nucleic acid samples of interest to provide a plurality of libraries of the nucleic acid samples, (b) ligating sequencing adapters carrying a 3′
-T overhang to the complexity reduced nucleic acid samples in the libraries and combining the libraries;(c) sequencing at least a portion of the combined libraries to obtain sequences, wherein the libraries are each tagged with an identifier sequence, and wherein the sequencing step comprises sequencing on a solid support; (d) aligning each of the sequences obtained in step (c) to obtain an alignment; and (e) determining one or more polymorphisms between the plurality of nucleic acid samples in the alignment of step (f).
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Specification