Methods for detecting target analytes and enzymatic reactions
First Claim
1. An assay kit comprising:
- an array substrate having a surface comprising a plurality of wells, wherein the wells are at a density of at least 10,000 sites per 1 mm2; and
a population of beads, wherein beads of said population are sized to fit within said wells and are functionalized with a nucleic acid, and wherein each well is dimensioned so as to accommodate not more than one bead of said population of beads, wherein the substrate is a fiber optic bundle.
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Abstract
A microsphere-based analytic chemistry system and method for making the same is disclosed in which microspheres or particles carrying bioactive agents may be combined randomly or in ordered fashion and dispersed on a substrate to form an array while maintaining the ability to identify the location of bioactive agents and particles within the array using an optically interrogatable, optical signature encoding scheme. A wide variety of modified substrates may be employed which provide either discrete or non-discrete sites for accommodating the microspheres in either random or patterned distributions. The substrates may be constructed from a variety of materials to form either two-dimensional or three-dimensional configurations. In a preferred embodiment, a modified fiber optic bundle or array is employed as a substrate to produce a high density array. The disclosed system and method have utility for detecting target analytes and screening large libraries of bioactive agents.
189 Citations
12 Claims
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1. An assay kit comprising:
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an array substrate having a surface comprising a plurality of wells, wherein the wells are at a density of at least 10,000 sites per 1 mm2; and a population of beads, wherein beads of said population are sized to fit within said wells and are functionalized with a nucleic acid, and wherein each well is dimensioned so as to accommodate not more than one bead of said population of beads, wherein the substrate is a fiber optic bundle.
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2. An assay kit comprising:
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an array substrate having a surface comprising a plurality of wells, wherein the wells are at a density of at least 10,000 sites per 1 mm2; and a population of beads, wherein beads of said population are sized to fit within said wells and are functionalized with a nucleic acid, and wherein each well is dimensioned so as to accommodate not more than one bead of said population of beads, wherein the wells are chemically altered. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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Specification