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Manipulation of beads in droplets and methods for manipulating droplets

  • US 9,377,455 B2
  • Filed: 06/22/2015
  • Issued: 06/28/2016
  • Est. Priority Date: 04/18/2006
  • Status: Active Grant
First Claim
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1. A method of circulating magnetically responsive beads within a droplet on a droplet actuator, the method comprising:

  • (a) providing a droplet actuator, comprising;

    (i) top and bottom substrates;

    (ii) a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable; and

    (iii) a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces;

    (b) positioning the droplet comprising magnetically responsive beads between the top and bottom substrates in a gap that is within the magnetic fields; and

    (c) selectively altering at least one of the magnetic fields to circulate the magnetically responsive beads in the droplet.

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