Manipulation of beads in droplets and methods for manipulating droplets
First Claim
1. A method of circulating magnetically responsive beads within a droplet on a droplet actuator, the method comprising:
- (a) providing a droplet actuator, comprising;
(i) top and bottom substrates;
(ii) a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable; and
(iii) a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces;
(b) positioning the droplet comprising magnetically responsive beads between the top and bottom substrates in a gap that is within the magnetic fields; and
(c) selectively altering at least one of the magnetic fields to circulate the magnetically responsive beads in the droplet.
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Abstract
The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.
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Citations
17 Claims
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1. A method of circulating magnetically responsive beads within a droplet on a droplet actuator, the method comprising:
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(a) providing a droplet actuator, comprising; (i) top and bottom substrates; (ii) a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable; and (iii) a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces; (b) positioning the droplet comprising magnetically responsive beads between the top and bottom substrates in a gap that is within the magnetic fields; and (c) selectively altering at least one of the magnetic fields to circulate the magnetically responsive beads in the droplet. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method of circulating magnetically responsive beads within a droplet on a droplet actuator, the method comprising:
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(a) providing a droplet actuator, comprising; (i) top and bottom substrates; (ii) a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable; and (iii) a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces; (b) positioning the droplet comprising magnetically responsive beads between the top and bottom substrates in a gap that is within the magnetic fields; and (c) selectively altering at least one of the magnetic fields to circulate the magnetically responsive beads in the droplet without splitting, merging or transporting the droplet. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17)
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Specification